Recombinant Human TSLP protein

• Cat.No.: TSLP-312H
• Product Overview: Recombinant Human TSLP protein was expressed in Escherichia coli.

Specification

• Description: Thymic stromal lymphopoietin (TSLP) is a hemopoietic protein belonging to the cytokine family and is known to play an important role in the maturation of T cell populations through activation of antigen presenting cells. It is mainly expressed in a number of tissues including heart, liver and prostate. TSLP signals through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. After binding STAT5 phosphorylation is induced resulting in the expression of downstream transcription factors. Like IL-7, TSLP induces phosphorylation of STAT3 and STAT5, but uses kinases other than the JAKs for activation. TSLP has the functions of enhancing the maturation of CD11c+ dendritic cells and inducing allergic inflammation by directly activating mast cells. It expression is linked to many disease states including asthma, inflammatory arthritis, atopic dermatitis, and eczema and other allergic states. But the factors inducing the activation of TSLP release are not clearly defined. Human TSLP shares approximately 43 % a.a. sequence identity with mouse TSLP.
• Source: E.coli
• Species: Human
• Form: Lyophilized from a 0.2μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
• Bio-activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-7Rα and human TSLP R co-transfected murine BaF3 pro-B cells is less than 0.3 ng/ml, corresponding to a specific activity of > 3.3 × 10⁶ IU/mg.
• Molecular Mass: Approximately 15.1 kDa, a single non-glycosylated polypeptide chain containing 132 amino acids.
• Protein length: 132
• AA Sequence: MYDFTNCDFEKIKAAYLSTISKDLI TYMSGTKSTEFNNTVSCSNRPHCLT EIQSLTFNPTAGCASLAKEMFAMKT KAALAIWCPGYSETQINATQAMKKR RKRKVTTNKCLEQVSQLQGLWRRFN RPLLKQQ
• Endotoxin: Less than 1 EU/μg of rHuTSLP as determined by LAL method.
• Purity: >98% by SDS-PAGE and HPLC analysis.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: TSLP
• Official Symbol: TSLP
• Synonyms: TSLP; thymic stromal lymphopoietin
• Gene ID: 85480
• mRNA Refseq: NM_033035
• Protein Refseq: NP_149024
• MIM: 607003
• UniProt ID: Q969D9

Reviews

04/04/2019

The TSLP protein offered by the manufacturer excels in its quality and is an excellent match for my experimental requirements.

02/25/2019

Their team of experts is readily available to address any queries or concerns I may have regarding the TSLP protein.

11/22/2016

Their team of experts is readily available to address any concerns or queries I may have regarding the TSLP protein.

Q&As

Can WDR5 play a role in non-cancerous diseases?

Yes, WDR5 has been associated with neurodevelopmental disorders, suggesting a role in non-cancerous diseases as well.

How does WDR5 affect neurodevelopment?

WDR5 regulates the expression of genes critical for neural development, and dysregulation can lead to developmental disorders.

Are there any clinical trials involving WDR5-targeted therapies?

Yes, there are ongoing clinical trials to test the safety and efficacy of WDR5-targeted therapies in cancer patients.

Is there a connection between WDR5 and autoimmune diseases?

Some studies suggest a potential link between WDR5 and autoimmune diseases, but further research is needed to confirm this association.

How can WDR5 be used as a diagnostic marker for cancer?

Elevated levels of WDR5 in certain cancers can serve as a diagnostic marker when detected in patient samples.