Recombinant Mouse Fgf15, His-tagged

• Cat.No.: Fgf15-499M
• Product Overview: Total 203 AA. MW: 23.78 kDa (calculated). N-Terminal His-tag, 10 extra AA (highlighted).

Specification

• Cat. No.: Fgf15-499M
• Description: Fibroblast growth factor 19 is a protein that in humans is encoded by the FGF19 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. This growth factor is a high affinity, heparin dependent ligand for FGFR4. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development.
• Source: E. coli
• Species: Mouse
• Form: Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 20mM NaCl, PH 8.0.
• Research topic: Energy metabolism and body weight regulation
• Purity: >90%
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: ELISA, Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein; LAL to determine quantity of endotoxin
• Sequences of amino acids: MKHHHHHHAS RPLAQQSQSV SDEDPLFLYG WGKITRLQYL YSAGPYVSNC FLRIRSDGSV DCEEDQNERN LLEFRAVALK TIAIKDVSSV RYLCMSADGK IYGLIRYSEE DCTFREEMDC LGYNQYRSMK HHLHIIFIQA KPREQLQDQK PSNFIPVFHR SFFETGDQLR SKMFSLPLES DSMDPFRMVE DVDHLVKSPS FQK
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Gene Information

• Gene Name: Fgf15 fibroblast growth factor 15 [ Mus musculus ]
• Official Symbol: Fgf15
• Synonyms: Fgf15; fibroblast growth factor 15; FGF19; FGF-15
• Gene ID: 14170
• mRNA Refseq: NM_008003
• Protein Refseq: NP_032029
• Pathway: Downstream signaling of activated FGFR; ESC Pluripotency Pathways; FGFR ligand binding and activation; FGFR1 ligand binding and activation; FGFR1b ligand binding and activation; FGFR1c and Klotho ligand binding and activation; FGFR2b ligand binding and activation; FRS2-mediated cascade; IRS-related events; MAPK signaling pathway; PI-3K cascade; Pathways in cancer; Regulation of actin cytoskeleton; Signaling by FGFR; Signaling by Insulin receptor; Insulin receptor signalling cascade
• Function: fibroblast growth factor receptor binding; growth factor activity

Reviews

11/16/2021

With its remarkable quality, reliability, and the manufacturer's excellent technical support, I am confident that it will be an invaluable asset in driving my scientific inquiries forward and facilitating groundbreaking discoveries in my field.

03/01/2020

This variety allows me to select the most appropriate option based on the specific requirements of my research.

07/16/2018

The manufacturer's commitment to quality control and rigorous testing guarantees the reliability and reproducibility of the FGF15 protein, which is essential for obtaining accurate and consistent results.

Q&As

In what ways does FGF15 contribute to the regulation of energy homeostasis?

FGF15 contributes to energy homeostasis by affecting nutrient absorption and metabolism in the small intestine and liver.

Are there ongoing clinical trials involving FGF15 in the treatment of metabolic disorders?

Yes, there are clinical trials investigating the use of FGF15 analogs or modulators for the treatment of metabolic disorders, including obesity and diabetes.

How does FGF15 impact glucose and lipid metabolism?

FGF15 plays a role in glucose and lipid metabolism by influencing the expression of genes involved in these processes in the liver.

How is FGF15 linked to the gut-liver axis, and why is it important in clinical research?

FGF15 is a key player in the gut-liver axis, influencing communication between the gut and the liver. Understanding this axis is crucial for developing targeted therapies for liver and metabolic diseases.

Are there any known side effects or risks associated with FGF15-based therapies?

The potential side effects and risks of FGF15-based therapies are still under investigation in clinical trials, and the safety profile is not fully established.