Recombinant Rat Cntfr protein(Met1-Pro346), His-tagged

• Cat.No.: Cntfr-4084R
• Product Overview: Recombinant Rat CNTFR (Q08406-1) (Met 1-Pro 346) was expressed in Insect Cells, fused with a C-terminal polyhistidine tag.

Specification

• Source: Insect Cells
• Species: Rat
• Tag: C-His
• Protein length: Met1-Pro346
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Rat CTNFR at 10 μg/ml (100 μl/well) can bind biotinylated human CNTF with a linear range of 1.28-160 ng/ml.
• Molecular Mass: The recombinant rat CNTFR comprises 356 amino acids with a predicted molecular mass of 39.6 kDa. It migrates as an approximately 48 kDa band in reduced SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cntfr ciliary neurotrophic factor receptor [ Rattus norvegicus ]
• Official Symbol: Cntfr
• Synonyms: CNTFR; ciliary neurotrophic factor receptor; ciliary neurotrophic factor receptor subunit alpha; CNTFR alpha; CNTFR-alpha; CNTF receptor subunit alpha; ciliary neurotrophic factor receptor alpha
• Gene ID: 313173
• mRNA Refseq: NM_001003929
• Protein Refseq: NP_001003929