Active Recombinant Rat Carboxypeptidase B1 (tissue)

• Cat.No.: Cpb1-1244R
• Product Overview: Recombinant Rat Cpb1 is expressed in E.Coli having a Mw of 35kDa is purified by standard chromatography techniques. Recombinant Rat Cpb1 is free from foreign enzymes such as carboxypeptidase A & chymotrypsin. Recombinant Cpb1 is free from protease inhibitors such as PMSF and EDTA.

Specification

• Cat. No.: Cpb1-1244R
• Description: Cpb1 (EC 3.4.17.2) catalyzes hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal end of polypeptides. The Mw was found to be 34.5 kda, optimun pH-7.9, and pI-6. Cpb1 is inhibited by arginine, lysine and ornithine. The enzyme is not inhibited by di-isopropylfluorophosphate (DFP), but it is inhibited by metal chelating agents, e.g., EDTA, 1,10-phenanthroline.
• Source: Rat
• Host: Escherichia Coli.
• Form: The protein was lyophilized with 100mM NaCl and 20mM Tris pH-7.5.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Bio-activity: 170 units/mg protein.
• Physical Appearance: Sterile Filtered lyophilized powder.
• Solubility: It is recommended to reconstitute the lyophilized Rat Cpb1 in sterile 18Mμ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Storage: Recombinant Rat Cpb1 although stable at room temp for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: Cpb1 carboxypeptidase B1 (tissue) [ Rattus norvegicus ]
• Official Symbol: Cpb1
• Synonyms: Cpb1; carboxypeptidase B1 (tissue); Cpb; carboxypeptidase B; pancreatic carboxypeptidase B1; EC 3.4.17.2; Pancreas-specific protein; CPB; PASP; PCPB; DKFZp779K1333; OTTHUMP00000215657; OTTHUMP00000215669; procarboxypeptidase B; EC 3.4.17
• Gene ID: 24271
• mRNA Refseq: NM_012533
• Protein Refseq: NP_036665
• UniProt ID: P19223
• Chromosome Location: 2q24
• Pathway: Pancreatic secretion
• Function: carboxypeptidase activity; metal ion binding; metallocarboxypeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding