Hepatitis B is one of a few known non-retroviralviruses which employ reverse transcriptionas a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA(pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA.
Mouse. Hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.
hepatitis virus granule
>90% pure (SDS-PAGE). Protein A chromatography
6.0mg/ml (OD280nm, E0.1% = 1.4)
Detection for HBe antigen. Suitable for use in ELISA, Western-Blotting and tissue immunity, Suggested pair for ELISA immunoassay:Capture DetectionCAB045 CAB046
Short term at 2-8℃,Long term at -20℃