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Cat. No.
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Aurka-989M
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Product Overview
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Recombinant
full-length mouse Aurka was expressed by baculovirus in Sf9 insect cells
using an N-terminal GST tag.
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Description
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Aurka belongs to a
multigenic family of mitotic serine/threonine kinases which are involved in
the control of chromosome segregation. Aurka is involved in centrosome
separation, duplication and maturation as well as in bipolar spindle assembly
and stability. Aurka is expressed and active at the highest level during G2-M
phase of the cell cycle. Overexpression of Aurka has been found to be correlated
with the grade of various human solid tumours. Ectopic Aurka overexpression
in any culture cell line leads to polyploidy and centrosome amplification.
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Applications
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Kinase Assay;
Western Blot
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Molecular Weight
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71 kDa
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Expression System
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Sf9 insect cells using baculovirus
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Species
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Mouse
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Form
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Recombinant protein
stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM
EDTA, 0.1 mM PMSF, 25 % glycerol.
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Specific Activity
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69 nmol/min/mg
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Purity
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>
90 %
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Concentration
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0.1 ug/ul
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Sequences
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Full-length
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Storage
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Store
product at -70oC. For optimal
storage, aliquot target into smaller quantities after centrifugation and
store at recommended temperature. For most favorable performance, avoid
repeated handling and multiple freeze/thaw cycles.
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Antigen Gene Information
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Gene Name
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Aurka aurora kinase A [ Mus
musculus ]
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Official Symbol
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Aurka
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Synonyms
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Aurka;
aurora kinase A; ARK1; IAK; Ark1; Ayk1; IAK1; Stk6; AIRK1; AU019385;
AW539821; Aurora-A; serine/threonine-protein kinase 6; ARK-1; aurora A;
aurora family kinase 1; aurora-related kinase 1; serine/threonine kinase 6;
aurora/IPL1-related kinase 1; ipl1- and aurora-related kinase 1; serine/threonine-protein
kinase Ayk1; serine/threonine-protein kinase aurora-A; Aurora family kinase 1;
Aurora kinase A; Aurora/IPL1-related kinase 1; Ipl1- and aurora-related
kinase 1; Serine/threonine-protein kinase Ayk1; Serine/threonine-protein
kinase aurora-A; EC=2.7.11.1;
OTTMUSP00000017294
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GeneID
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20878
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mRNA Refseq
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NM_011497
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Protein Refseq
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NP_035627
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UniProt ID
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Q3TEY6
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Chromosome Location
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2 H3; 2
100.0 cM
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Pathways
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APC/C-mediated
degradation of cell cycle proteins; Cell Cycle; Oocyte meiosis; Regulation of
mitotic cell cycle; APC/C:Cdh1 mediated degradation of Cdc20 and other
APC/C:Cdh1 targeted proteins in late mitosis/early G1
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Function
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ATP
binding; kinase activity; nucleotide binding; protein binding; protein kinase
activity; protein kinase binding; protein serine/threonine kinase activity; transferase
activity; transferase activity, transferring phosphorus-containing groups;
ubiquitin protein ligase binding
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.jpg)
Protein AURKA PDB 1mq4.
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Download Datasheet:
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