Recombinant Human KIT v-kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog, GST-tagged

• Cat.No.: KIT-4857H
• Product Overview: Recombinant human KIT (544-end) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Specification

• Cat. No.: Kit-4857H
• Description: KIT is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed curbing neoplastic mast cell growth.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 73 kDa
• Expression System: Sf9 insect cells using baculovirus.
• Species: Human
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM EDTOA, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
• Purity: > 85 %
• Concentration: 0.1 ug/ul
• Sequences: 544-end
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Acute myeloid leukemia; Acute myeloid leukemia; C-MYB transcription factor network; Cytokine-cytokine receptor interaction; Endocytosis; Hematopoietic cell lineage; Kit Receptor Signaling Pathway; Melanogenesis; Pathways in cancer; Signaling events mediated by Stem cell factor receptor (c-Kit)

Gene Information

• Gene Name: KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog [ Homo sapiens ]
• Official Symbol: KIT
• Synonyms: KIT; PBT; SCFR; C-Kit; CD117; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; EC 2.7.10.1; piebald trait; mast/stem cell growth factor receptor; proto-oncogene tyrosine-protein kinase Kit; soluble KIT variant 1; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein; CD117 antigen; EC 2.7.10
• Gene ID: 3815
• mRNA Refseq: NM_000222
• Protein Refseq: NP_000213
• MIM: 609132
• UniProt ID: P10721
• Chromosome Location: 4q11-q12
• Function: ATP binding; cytokine binding; nucleotide binding; protein binding; protein homodimerization activity; protein tyrosine kinase activity; receptor activity; receptor signaling protein tyrosine kinase activity; stem cell factor receptor activity; transmembrane receptor protein tyrosine kinase activity