Recombinant Human Long Arg3 Insulin-like Growth Factor-I
Cat.No. : | IGF-08H |
Product Overview : | Recombinant Human Long-R3-IGF-I produced inE. coliis a single, non-glycosylated, polypeptide chain containing 83 amino acids and having a molecular mass of 9.111 kDa. |
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Cat. No. : | IGF-08H |
Description : | Human Long Arg3Insulin-like Growth Factor-I (Long-R3-IGF-I) is an 83 amino acid analog of human IGF-I comprising the complete human IGF-I sequence with the substitution of an Arg for the Glu at position 3 (hence R3), and a 13 amino acid extension peptide at the N-terminus. Scientists have engineered this analog with the express purpose of increasing biological activity. Human Long-R3-IGF-I is significantly more potent than human IGF-I in vitro. The enhanced potency is due to the markedly decreased binding of human Long-R3-IGF-I to IGF binding proteins which normally inhibit the biological actions of IGFs. |
Purity : | >95% by SDS-PAGE and RP-HPLC. |
Formulation : | Lyophilized after extensive dialysis against acetic acid buffer. |
Specific Activity : | The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of 1.0 x 106IU/mg. For most in-vitro applications, Long-R3-IGF-I exerts its biological activity in the concentration range of 0.2-20 ng/ml. |
Endotoxin : | Less than 0.1ng/µg (1IEU/µg) of Long-R3-IGF-I. |
Reconstitutionon : | It is recommended to reconstitute the lyophilized Long-R3-IGF-I in 500mM acetic acid not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storagee : | Lyophilized Long-R3-IGF-I although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Long-R3-IGF-I should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles. |
Gene Name : | IGF1 |
Synonyms : | IBP1; IGF-IA; IGF-IB; IGFI; MGF; Somatomedin-C; Insulin-like growth factor IA precursor; Insulin-like growth factor IB precursor; Mechano growth factor; insulin-like growth factor 1; IGF1; insulin-like growth factor 1 (somatomedin C) |
Gene ID : | 3479 |
mRNA Refseq : | NM_000618.3 |
Protein Refseq : | NP_000609.1 |
MIM : | 147440 |
UniProt ID : | P01343; P05019 |
Chromosome Location : | 12q22-q23 |
Pathway : | Focal adhesion, Glioma, Long-term depression, Melanoma, Prostate cancer, mTOR signaling pathway |
Function : | Growth factor activity, Hormone activity, Insulin-like growth factor receptor binding, Prothoracicotrophic hormone activity |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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