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Recombinant Human IGFBP6 protein

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Cat.No.:
IGFBP6-5053H
Product Overview:
Recombinant Human IGFBP6 was expressed in Insect cells.
Description:
IGF-BPs controls the distribution, function and activity of IGFs in various cell tissues and body fluids. IGF-BP6 is produced by bone cells and is the major IGF-BP present in cerebrospinal fluid, and specifically inhibits IGF-II actions. IGF-BP6 has been shown to inhibit IGF-II-dependent cancers such as neuroblastoma, colon cancer and rhabdomyosarcoma.
Source:
Insect cells
Species:
Human
Bio-activity:
Determined by its ability to inhibit IGF-II induced proliferation of human MCF-7 cells. The expected ED50 for this effect is 0.1 – 0.4 μg/ml.
Molecular Mass:
Recombinant human IGF-BP6 has a calculated mass of 22.6 kDa and consists of 213 amino acid residues including the IGF-BP domain and thyroglobulin type-I domain. IGF-BP6 migrates at an apparent molecular weight of approximately 23.0-30.0 kDa by SDS-PAGE analysis under non-reducing conditions.
AA Sequence:
RCPGCGQGV QAGCPGGCV EEEDGGSPA EGCAEAEGC LRREGQECG VYTPNCAPG LQCHPPKDD EAPLRALLL GRGRCLPAR APAVAEENP KESKPQAGT ARPQDVNRR DQQRNPGTS TTPSQPNSA GVQDTEMGP CRRHLDSVL QQLQTEVYR GAQTLYVPN CDHRGFYRK RQCRSSQGQ RRGPCWCVD RMGKSLPGS PDGNGSSSC PTGSSG
Purity:
Above 95% as determined by SDS-PAGE Analysis.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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