Recombinant Human CLU, Flag-tagged

• Cat.No.: CLU-106H
• Product Overview: The Clusterin Human contains a total 438 amino acids and a calculated molecular mass of 51.27kDa (calculated). The AA sequence (AA 1-427) is identical to Swiss-Prot-P10909 (AA 23-449, secreted Human Clusterin). C-terminal Flag-tag 11 extra AA (underlined).

Specification

• Description: The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.
• Source: 293 cell line (Human embryonic kidney)
• Species: Human
• Tag: Flag
• Form: Filtered, White, Lyophilized powder. Filtered (0.4 micron) and lyophilized PBS, pH 7.5.
• Molecular Mass: 51.27kDa
• AA Sequence: DQTVSDNELQ EMSNQGSKYV NKEIQNAVNG VKQIKTLIEK TNEERKTLLS NLEEAKKKKE DALNETRESE TKLKELPGVC NETMMALWEE CKPCLKQTCM KFYARVCRSGS GLVGRQLEE FLNQSSPFYF WMNGDRIDSL LENDRQQTHM LDVMQDHFSRA SSIIDELFQ DRFFTREPQD TYHYLPFSLP HRRPHFFFPK SRIVRSLMPF SPYEPLNFHA MFQPFLEMIH EAQQAMDIHF HSPAFQHPPT EFIREGDDDR TVCREIRHNS TGCLRMKDQC DKCREILSVD CSTNNPSQAKLRRELDESLQ VAERLTRKYN ELLKSYQWKM LNTSSLLEQL NEQFNWVSRL ANLTQGEDQYYLRVTTVASH TSDSDVPSGV TEVVVKLFDS DPITVTVPVE VSRKNPKFME TVAEKALQEY RKKHREEAAA DYKDDDDK.
• Purity: Greater than 95% as determined by SDS PAGE.
• Storage: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product not sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.

Gene Information

• Gene Name: CLU clusterin [ Homo sapiens (human) ]
• Official Symbol: CLU
• Synonyms: CLU; CLI; AAG4; APOJ; CLU1; CLU2; KUB1; SGP2; APO-J; SGP-2; SP-40; TRPM2; TRPM-2; NA1/NA2; clusterin; apolipoprotein J; ku70-binding protein 1; sulfated glycoprotein 2; aging-associated protein 4; complement lysis inhibitor; complement cytolysis inhibitor; complement-associated protein SP-40,40; testosterone-repressed prostate message 2
• Gene ID: 1191
• mRNA Refseq: NM_001831
• Protein Refseq: NP_001822
• MIM: 185430
• UniProt ID: P10909
• Chromosome Location: 8p21-p12
• Pathway: Platelet activation; Platelet degranulation; Response to elevated platelet cytosolic Ca2+
• Function: NOT ATPase activity; misfolded protein binding; ubiquitin protein ligase binding

Reviews

11/30/2021

Impressed with the quality control. This product is a gem in our lab experiments.

01/22/2019

Smooth transactions and swift delivery. Our lab supplies are incomplete without this protein.

04/17/2018

Our lab manager introduced us to this product, and we're never going back. Essential for our studies.

Q&As

How does CLU contribute to the regulation of inflammation, and what role does it play in immune responses and autoimmunity?

CLU has anti-inflammatory properties and modulates immune responses. Its involvement in autoimmunity is linked to its ability to regulate immune cell functions and cytokine production.

How does CLU contribute to the regulation of cell survival and apoptosis, and what are the molecular mechanisms involved?

CLU plays a role in regulating cell survival and apoptosis by interacting with key cellular components, including Bcl-2 family members and caspases, modulating apoptotic pathways.

How does CLU influence cell adhesion and migration, and what role does it play in tissue remodeling and repair processes?

CLU modulates cell adhesion and migration, contributing to tissue remodeling and repair. Its interactions with extracellular matrix components influence cellular behaviors in wound healing and tissue homeostasis.

Can you discuss the association between CLU and neurodegenerative disorders, and how does it impact the pathogenesis of conditions like Alzheimer's disease?

CLU is implicated in neurodegenerative disorders, particularly Alzheimer's disease. Its involvement in processes such as amyloid-beta aggregation and clearance influences disease progression.

Can you elaborate on the molecular interactions of CLU with lipids and its involvement in lipid transport and metabolism?

CLU interacts with lipids, participating in lipid transport and metabolism. Its role in lipoprotein remodeling and cholesterol homeostasis links it to cardiovascular health.