Recombinant Human METAP1, His-tagged

• Cat.No.: METAP1-15849H
• Product Overview: Recombinant human METAP1 protein, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: METAP1 (Methionine aminopeptidase 1) belongs to the peptidase M24A family. This protein removes the amino-terminal methionine from nascent proteins. It required for normal progression through the cell cycle. The active site of MetAP-1 contains two adjacent divalent metal ions connected by a water molecule or hydroxide ion.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 3mM DTT, 40% glycerol, 200mM NaCl, 0.1mM PMSF
• Molecular Mass: 45.7kDa (409aa), confirmed by MALDI-TOF
• Protein length: 1-386aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAAVETR VCETDGCSSE AKLQCPTCIK LGIQGSYFCS QECFKGSWAT HKLLHKKAKD EKAKREVSSW TVEGDINTDP WAGYRYTGKL RPHYPLMPTR PVPSYIQRPD YADHPLGMSE SEQALKGTSQ IKLLSSEDIE GMRLVCRLAR EVLDVAAGMIKPGVTTEEID HAVHLACIAR NCYPSPLNYY NFPKSCCTSV NEVICHGIPD RRPLQEGDIV NVDITLYRNG YHGDLNETFF VGEVDDGARK LVQTTYECLM QAIDAVKPGV RYRELGNIIQ KHAQANGFSV VRSYCGHGIH KLFHTAPNVP HYAKNKAVGV MKSGHVFTIE PMICEGGWQDETWPDGWTAV TRDGKRSAQF EHTLLVTDTG CEILTRRLDS ARPHFMSQF
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term. For long term storage, aliquot and store at at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: METAP1 methionyl aminopeptidase 1 [ Homo sapiens ]
• Official Symbol: METAP1
• Synonyms: METAP1; methionyl aminopeptidase 1; methionine aminopeptidase 1; KIAA0094; MAP1A; MetAP1A; Peptidase M; MAP 1; metAP 1; peptidase M 1; DKFZp781C0419
• Gene ID: 23173
• mRNA Refseq: NM_015143
• Protein Refseq: NP_055958
• MIM: 610151
• UniProt ID: P53582
• Chromosome Location: 4q23
• Function: aminopeptidase activity; metal ion binding; metalloexopeptidase activity; peptidase activity