Recombinant Human ADD2, His-tagged

• Cat.No.: ADD2-9414H
• Product Overview: Recombinant Human ADD2 protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Description: Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described.
• Source: E.coli
• Species: Human
• Tag: His
• Protein length: 1-331a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.

Gene Information

• Gene Name: ADD2 adducin 2 (beta) [ Homo sapiens ]
• Official Symbol: ADD2
• Synonyms: ADD2; adducin 2 (beta); beta-adducin; ADDB; erythrocyte adducin subunit beta
• Gene ID: 119
• mRNA Refseq: NM_001185054
• Protein Refseq: NP_001171983
• MIM: 102681
• UniProt ID: P35612
• Chromosome Location: 2p13.3
• Function: actin binding; actin filament binding; calmodulin binding; metal ion binding; protein heterodimerization activity; protein homodimerization activity; spectrin binding; structural molecule activity