Active Recombinant Human AGA protein, His-tagged

• Cat.No.: AGA-135H
• Product Overview: Recombinant Human AGA protein(Met1-Ile346)(CAA39029.1), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to hydrolyze the AspAMC. The specific activity is >300 pmol/min/μg.
• Molecular Mass: The recombinant human AGA comprises 334 amino acids and has a predicted molecular mass of 36.1 kDa. The apparent molecular mass of the protein is approximately 47, 29, 23 and 20 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Protein Length: Met1-Ile346
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: AGA aspartylglucosaminidase [ Homo sapiens ]
• Official Symbol: AGA
• Synonyms: AGA; aspartylglucosaminidase; N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase; ASRG; glycosylasparaginase; aspartylglucosylamine deaspartylase; N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase; GA; AGU
• Gene ID: 175
• mRNA Refseq: NM_000027
• Protein Refseq: NP_000018
• MIM: 613228
• UniProt ID: P20933

Reviews

11/04/2018

 The product’s adaptability to various buffers contributed to its stable and consistent performance.

03/18/2018

The consistent performance of the product simplified data interpretation.

11/26/2017

 Our research was fortified by the product’s consistent behavior, fostering robust results.

01/12/2017

The high-quality Protein AGA enabled precise quantification of target proteins. 

12/06/2016

Our assays showed a significant increase in enzyme activity using Ag5.

Q&As

Where is the AGA protein found?

The AGA protein is found in lysosomes, which are cell organelles involved in breaking down cellular waste.

How did researchers study AGA protein phosphorylation?

Researchers used the PhosphoSitePlus tool and molecular dynamics simulations to analyze AGA protein phosphorylation.

How is AGA protein’s interaction with glycoprotein substrates studied

Research investigates how AGA protein’s specificity for aspartylglucosamine residues influences substrate interactions.

How does AGA protein contribute to lysosomal homeostasis

AGA protein’s role contributes to maintaining lysosomal homeostasis and overall cellular health.

What happens if the AGA protein is deficient?

Deficiency of the AGA protein leads to Aspartylglucosaminuria, a rare genetic disorder characterized by the buildup of glycoproteins in the body.

What was observed in the C163S mutant AGA protein?

The C163S mutant AGA protein exhibited increased hydrogen bonds and greater compactness compared to the wild type.

Is AGA protein’s function connected to lysosomal pH regulation

Yes, AGA protein’s activity might play a role in maintaining optimal lysosomal pH for enzymatic activity.

How does AGA protein’s involvement relate to lysosomal enzyme trafficking

AGA protein’s role may include participating in lysosomal enzyme trafficking to enhance degradation processes.

What structural changes occurred in phosphorylated AGA forms?

Phosphorylated AGA forms (Y178-p, T215-p, T324-p) showed increased intramolecular hydrogen bonds and greater compactness.

Can AGA protein’s function affect lysosomal enzyme activity

Yes, AGA protein’s function impacts lysosomal enzyme activity and contributes to efficient degradation.

What does the AGA protein do?

The AGA protein catalyzes the hydrolysis of N-linked glycoproteins, breaking them down into smaller components.

What is the function of the AGA protein?

The AGA protein plays a role in the metabolism of glycoproteins.

Is AGA protein’s activity regulated by cellular conditions

Yes, AGA protein’s activity can be influenced by cellular conditions and the presence of its substrates.

How is AGA protein’s impact on cellular responses to glycoprotein buildup explored

Research aims to uncover how AGA protein’s function mitigates cellular responses to glycoprotein accumulation.

Can AGA protein’s function be linked to glycoprotein metabolism

Yes, AGA protein’s role directly influences glycoprotein metabolism and turnover within cells.

Does AGA protein play a role in lysosomal exocytosis

Yes, AGA protein’s involvement can extend to lysosomal exocytosis and the release of lysosomal contents.

How is AGA protein’s involvement studied in lysosomal disorders

Research investigates AGA protein’s role in lysosomal storage disorders, aiding in understanding disease mechanisms.

What happens in Aspartylglucosaminuria (AGU)?

In AGU, insufficient AGA activity leads to the buildup of glycoproteins, causing chronic neurodegeneration.

How does AGA protein affect cellular responses to lysosomal stress

AGA protein’s function could impact cellular responses to lysosomal stressors and maintain lysosomal function.

Can AGA protein’s role contribute to lysosome-related diseases

Yes, AGA protein’s malfunction can lead to lysosomal storage disorders, disrupting glycoprotein degradation.