Recombinant Human SPHK1, His-GST tagged

• Cat.No.: SPHK1-277H
• Product Overview: Recombinant Human SPHK1 (Q9NYA1-1) (Met1-Leu384), fused with the N-terminal polyhistidine-tagged GST tag, was produced in Baculovirus-Insect cells.

Specification

• Source: Baculovirus-Insect cells
• Species: Human
• Tag: His-GST
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 8.0.Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant human SPHK1 consists of 621 amino acids and predicts a molecular mass of 70.3 KDa. The apparent molecular mass of the protein is approximately 64 KDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: SPHK1 sphingosine kinase 1 [ Homo sapiens ]
• Official Symbol: SPHK1
• Gene ID: 8877
• mRNA Refseq: NM_001142601
• Protein Refseq: NP_001136073
• MIM: 603730
• UniProt ID: Q9NYA1
• Chromosome Location: 17q25.2