Active Recombinant Human BTK protein, His-tagged

• Cat.No.: BTK-398H
• Product Overview: Recombinant Human BTK protein(NP_000052.1)(Met 1-Ser 659), fused with His tag, was expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.0.Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: The specific activity was determined to be >50 nmol/min/mg using Poly(Glu,Tyr)4:1 peptide as substrate.
• Molecular Mass: The secreted recombinant human BTK consists of 670 amino acids and predicts a molecular mass of 77.8 KDa.
• Protein Length: Met 1-Ser 659
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: BTK Bruton agammaglobulinemia tyrosine kinase [ Homo sapiens ]
• Official Symbol: BTK
• Synonyms: BTK; Bruton agammaglobulinemia tyrosine kinase; AGMX1, IMD1; tyrosine-protein kinase BTK; ATK; PSCTK1; XLA; B-cell progenitor kinase; agammaglobulinaemia tyrosine kinase; tyrosine-protein kinase BTK isoform (lacking exon 14); dominant-negative kinase-deficient Brutons tyrosine kinase; AT; BPK; IMD1; AGMX1; MGC126261; MGC126262
• Gene ID: 695
• mRNA Refseq: NM_000061
• Protein Refseq: NP_000052
• MIM: 300300
• UniProt ID: Q06187

Reviews

02/08/2022

High purity, lack of impurities, in the experiment performance is very efficient.

02/03/2022

Short half-life, more flexible dosage plan.

09/12/2021

It's stable in all kinds of environments.

Q&As

What is the current progress in drug development for BTK?

The development of drugs targeting BTK is currently in the clinical trial stage, including small molecule inhibitors against BTK and inhibitors targeting BTK signaling pathways.

How can BTK be used as a biomarker in clinical practice?

In clinical practice, the expression level of BTK can be detected to predict disease progression and prognosis, and to guide the choice of treatment regimen. For example, in the diagnosis and treatment of lymphoma and leukemia, testing the expression level of BTK can help doctors determine the type, stage and prognosis of the disease, and guide the choice of treatment regimen.

What is the significance of studying BTK proteins?

BTK proteins can help to gain insight into the mechanisms of B cell development and activation, as well as the pathogenesis of certain diseases such as lymphoma and leukemia. In addition, it is of great significance for the development of new drugs and treatment strategies.

Does BTK protein play a role in tumor immunotherapy?

BTK protein plays an important role in tumor immunotherapy, which can activate the immune response of tumor cells, promote apoptosis of tumor cells and inhibit the proliferation of tumor cells.

How is BTK protein related to viral infection?

Studies have shown that BTK protein can participate in the regulation of antiviral immune responses and affect the course of viral infection.

What are the applications of BTK protein in drug discovery?

It can be used as a target for the development of new drugs, or as a drug carrier for the directional delivery of drugs.