| Description : |
Cerebral dopamine neurotrophic factor (CDNF), also known as ARMET-like protein 1, is encoded by the CDNF gene in humans and widely expressed in neuronal and non-neuronal tissues. The CDNF is a novel neurotrophic factor with strong trophic activity on dopaminergic neurons comparable to that of glial cell line-derived neurotrophic factor (GDNF). Furthermore, it also prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. By research, CDNF might be beneficial for the treatment of Parkinson"s disease. Recombinant human CDNF contains 162 amino acid residues and it shares 81 % and 84 % a.a. sequence identity with murine and rat CDNF. |
| Source : |
E. coli |
| Species : |
Human |
| Form : |
Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM PB, 150 mM NaCl, pH 6.4, 3% trehalose. |
| Bio-activity : |
Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using rat C6 cells is less than 25 μg/ml, corresponding to a specific activity of >4 × 10^4 IU/mg. |
| Molecular Mass : |
18.3 kDa |
| AA Sequence : |
QEAGGRPGAD CEVCKEFLNR FYKSLIDRGV NFSLDTIEKE LISFCLDTKG KENRLCYYLG ATKDAATKIL SEVTRPMSVH MPAMKICEKL KKLDSQICEL KYEKTLDLAS VDLRKMRVAE LKQILHSWGE ECRACAEKTD YVNLIQELAP KYAATHPKTE L |
| Endotoxin : |
Less than 1 EU/μg of rHuCDNF as determined by LAL method. |
| Purity : |
>96% by SDS-PAGE and HPLC analyses. |
| Usage : |
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| Storage : |
This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles. |
| Reconstitution : |
We recommend that this vial be briefy centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
