Recombinant Human UBE2N, His-tagged

• Cat.No.: UBE2N-238H
• Product Overview: Recombinant human UBE2N (UBC13) (2-end) was expressed in E. coli cells using an N-terminal His tag.

Specification

• Description: UBE2N (UBC13) or ubiquitin-conjugating enzyme E2N is a member of the E2 ubiquitin-conjugating enzyme family which plays a role in DNA post-replication repair. UBE2N gene encodes a novel human E2 that may be involved in protein degradation in the muscles and testis. UBE2N plays a role in initiation of homologous recombination during double-strand break repair. UBE2N activity may have therapeutic uses in controlling inflammatory responses and it is also important in the induction of immune responses.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Molecular Mass: ~18 kDa
• Purity: >95% by densitometry
• Applications: Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: UBE2N ubiquitin-conjugating enzyme E2N [ Homo sapiens ]
• Official Symbol: UBE2N
• Synonyms: UBE2N; ubiquitin-conjugating enzyme E2N; ubiquitin conjugating enzyme E2N (homologous to yeast UBC13) , ubiquitin conjugating enzyme E2N (UBC13 homolog, yeast); ubiquitin-conjugating enzyme E2 N; MGC8489; UBC13; UbcH ben; yeast UBC13 homolog; ubiquitin-protein ligase N; ubiquitin carrier protein N; bendless-like ubiquitin conjugating enzyme; bendless-like ubiquitin-conjugating enzyme; ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13); UbcH-ben; MGC131857
• Gene ID: 7334
• mRNA Refseq: NM_003348
• Protein Refseq: NP_003339
• MIM: 603679
• UniProt ID: P61088
• Chromosome Location: 12q22
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination & Proteasome degradation, organism-specific biosystem; Antiviral mechanism by IFN-stimulated genes, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem
• Function: ATP binding; acid-amino acid ligase activity; ligase activity; nucleotide binding; protein binding; ubiquitin binding; ubiquitin-protein ligase activity