Active Recombinant Canine ERBB2, His-tagged

• Cat.No.: ERBB2-171C
• Product Overview: A DNA sequence encoding the canine ERBB2 (ABW99108.1) (Met1-Thr652) was expressed with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Canine
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: Immobilized Her2/ERBB2 Protein, Canine, Recombinant (His Tag) at 2 μg/ml (100 μl/well) can bind Anti-Erbb2 Antibody (Trastuzumab), the EC50 is 8-50 ng/mL.
• Molecular Mass: The recombinant canine ERBB2 comprises 641 amino acids and has a predicted molecular mass of 70.4 kDa. The apparent molecular mass of the protein is approximately 75- 95 kDa in SDS-PAGE under reducing conditions.
• Protein Length: Met1-Thr652
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.