Recombinant Mouse PGK1 cell lysate

• Cat.No.: PGK1-547MCL
• Product Overview: Mouse PGK1 / PGKA derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Baculovirus-Insect Cells
• Species: Mouse
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: Pgk1 phosphoglycerate kinase 1 [ Mus musculus ]
• Official Symbol: PGK1
• Synonyms: PGK1; phosphoglycerate kinase 1; Pgk-1; MGC118097
• Gene ID: 18655
• mRNA Refseq: NM_008828
• Protein Refseq: NP_032854
• Pathway: Gluconeogenesis, organism-specific biosystem; Gluconeogenesis, oxaloacetate =>fructose-6P, organism-specific biosystem; Gluconeogenesis, oxaloacetate => fructose-6P, conserved biosystem; Glucose metabolism, organism-specific biosystem; Glycolysis, organism-specific biosystem
• Function: ADP binding; ATP binding; kinase activity; nucleotide binding; phosphoglycerate kinase activity; phosphoglycerate kinase activity; transferase activity

Reviews

03/14/2020

manufacturers may offer customization options, allowing researchers to tailor the PGK1 protein to their specific needs.

02/27/2020

This may include options like different formulations, protein modifications, or tagging techniques. Customization can enhance the versatility of the protein and optimize its utility in the intended research.

04/15/2016

They can also address any questions or concerns regarding the PGK1 protein, thereby facilitating smooth and efficient research execution.

Q&As

How is PGK1 linked to neurological disorders?

PGK1 dysfunction has been associated with neurological conditions like Alzheimer's disease and Parkinson's disease due to its impact on cellular energy metabolism.

In what way does PGK1 affect cancer?

PGK1 is often upregulated in cancer cells, aiding their rapid growth and proliferation by enhancing glycolysis, known as the Warburg effect.

Can PGK1 be a target for cancer treatment?

Research suggests that targeting PGK1 could potentially be a strategy for cancer therapy by disrupting the energy production in cancer cells.

Are there any PGK1 inhibitors or activators in development for clinical use?

Several compounds that modulate PGK1 activity are being studied, but none have reached widespread clinical use yet.

Are there diagnostic uses for PGK1 in cancer?

Yes, elevated levels of PGK1 have been identified in certain cancers, making it a potential biomarker for cancer detection and prognosis.