Recombinant Human GALK1 cell lysate

• Cat.No.: GALK1-558HCL
• Product Overview: Human GALK1 / Galactokinase / Galactose kinase derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Baculovirus-Insect Cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: GALK1 galactokinase 1 [ Homo sapiens ]
• Official Symbol: GALK1
• Synonyms: GALK1; galactokinase 1; GALK; galactokinase; galactose kinase; GK1
• Gene ID: 2584
• mRNA Refseq: NM_000154
• Protein Refseq: NP_000145
• MIM: 604313
• UniProt ID: P51570
• Chromosome Location: 17q23-q25
• Pathway: Amino sugar and nucleotide sugar metabolism, organism-specific biosystem; Amino sugar and nucleotide sugar metabolism, conserved biosystem; Galactose catabolism, organism-specific biosystem; Galactose metabolism, organism-specific biosystem; Galactose metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem
• Function: ATP binding; galactokinase activity; galactose binding; kinase activity; nucleotide binding; transferase activity

Reviews

09/06/2022

The production process of GALK1 is stable and reliable, with high batch refolding.

07/21/2022

GALK1 is pure and has no other proteins or impurities.

04/28/2021

GALK1 has good stability in solution and is not easy to aggregate or deactivate.

Q&As

Are there different isoforms or variants of GALK1?

Yes, there may be different subtypes or variants of GALK1 that may have different biological functions.

How do these different subtypes or variants function in cells?

Different subtypes or variants may play different roles in cells, and the specific mechanisms need further research.

What are the effects of regulating GALK1 expression on cells?

Regulating the expression of GALK1 may affect the glycolysis process and energy metabolism of cells.

What is the mechanism of action of these drugs?

The mechanism of action of these drugs may include inhibition of GALK1 activity, regulation of its expression level, etc.

Is GALK1 involved in other biological processes?

In addition to glycolysis, GALK1 may also be involved in other biological processes, such as cell signaling.

How to regulate the expression of GALK1 experimentally?

The expression of GALK1 can be regulated by gene knockout, transgene and other technologies.