Recombinant Human APLP1 cell lysate

• Cat.No.: APLP1-1031HCL
• Product Overview: Human APLP1 / Amyloid-like derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: APLP1 amyloid beta (A4) precursor-like protein 1 [ Homo sapiens ]
• Official Symbol: APLP1
• Synonyms: APLP1; amyloid beta (A4) precursor-like protein 1; amyloid-like protein 1; amyloid precursor like protein 1; amyloid like protein 1; APLP; APLP-1; amyloid precursor-like protein 1
• Gene ID: 333
• mRNA Refseq: NM_001024807
• Protein Refseq: NP_001019978
• MIM: 104775
• UniProt ID: P51693
• Chromosome Location: 19q
• Function: alpha-2A adrenergic receptor binding; alpha-2B adrenergic receptor binding; alpha-2C adrenergic receptor binding; heparin binding; identical protein binding; protein binding; transition metal ion binding

Reviews

12/12/2022

Labeling effect in Western blot is very good, and operations such as fluorescent labeling can be performed to facilitate subsequent detection and analysis.

06/05/2022

Production process of APLP1 pays attention to harmonious coexistence with nature, and realizes zero pollution in the production process, demonstrating its persistent pursuit of environmental protection.

06/23/2020

The same batch of experiments are very similar, and the reproducibility is very high.

05/05/2020

Preparation process is strictly controlled to ensure the quality and safety of the product.

Q&As

Can recombinant APLP1 be used in the treatment of neurodegenerative diseases?

APLP1 is considered as one of the therapeutic directions for neurodegenerative diseases, and related research and development is ongoing.

What is the relationship between APLP1 and the formation of neuronal axons?

APLP1 is closely related to the formation of neuronal axons, which can regulate the formation and development of neuronal axons.

What role does APLP1 play in synaptic plasticity?

APLP1 plays an important role in synaptic plasticity, regulating the formation and plasticity of synapses.

What is the relationship between APLP1 and Alzheimer's disease?

Studies have shown that APLP1 is associated with Alzheimer's disease and may be one of the triggers of Alzheimer's disease.

What is the relationship between APLP1 and beta-amyloid protein?

APLP1 is related to beta-amyloid protein, which is one of the main components of beta-amyloid substances.

What is the physiological function of APLP1 ?

The physiological functions of APLP1 include cell signaling, neuronal development, neuronal protection, metabolic regulation and so on.

Is the genetic mutation of APLP1 associated with certain diseases?

Mutations in the APLP1 are associated with a variety of diseases, including cancer and neurological disorders.

What is the relationship between APLP1 and synaptic repair?

APLP1 is associated with the repair of neuronal synapses and can promote the reconstruction and repair of neuronal synapses.

Can APLP1 be used as a target for drug development?

It can be used as a target for drug development, and drug studies targeting APLP1 are ongoing.

What kind of diseases can APLP1 be used to treat?

APLP1 can be used in the treatment of neurodegenerative diseases, stroke, metabolic diseases and other aspects.

What is the role of APLP1 in cell signaling?

APLP1 plays an important role in cell signaling and can participate in a series of biological processes such as cell proliferation, apoptosis and differentiation.

What is the relationship between APLP1 and neuron cell membrane?

APLP1 can participate in the formation and stability of neuron cell membrane, and plays an important role in the development and maintenance of cell membrane.

In what fields does APLP1 have application prospects?

APLP1 can be applied to neurodegenerative diseases, cancer, and metabolic diseases.

Does APLP1 affect cognition and learning ability?

Some studies suggest that APLP1 may have an effect on memory and learning ability.

What is the relationship between APLP1 and cancer?

APLP1 has been found to be associated with a variety of cancers, and it may serve as a cancer marker or therapeutic target.

What are the antioxidant properties of APLP1 ?

APLP1 can play an antioxidant role by inhibiting the generation of free radicals and regulating the activity of antioxidant enzymes.

What is the role of APLP1 in inflammatory response?

APLP1 plays an important biological role in inflammatory response, and studies have shown that it can be involved in regulating the body's immune response and cytokine play.

What are the preparation methods of APLP1 ?

The preparation methods of APLP1 include genetic engineering technology, cell culture technology, etc.

What are the effects of APLP1 on adult nerve cells?

Studies have shown that APLP1 can improve the living environment of adult nerve cells and protect nerve cells from damage.

What is the role of APLP1 in nerve cells?

Studies have shown that APLP1 plays an important role in the development and protection of neurons and can prevent neuronal apoptosis.