Mouse anti- glutathione S-transferase Monoclonal Antibody

• Cat.No.: CAB103

Specification

• Cat. No.: CAB103
• Antigen information: The glutathione S-transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are capable of multiple reactions with a multitude of substrates, both endogenous and xenobiotic. This is a member of MAPEG family of transmembrane protein. These enzymes can constitute up to 10% of cytosolic protein in some mammalian organs. GSTs catalyse the conjugation of reduced glutathione via the sulfhydryl group, to electrophilic centers on a wide variety of substrates. This activity is useful in the detoxification of endogenous compounds such as peroxidised lipids, as well as the metabolism of xenobiotics. As well as their enzymatic activities, GSTs may also bind toxins and function as transport proteins. Because of this, an early term for GSTs was "ligandin".
• Specificity: Recognizes GST and GST-tagged fusion proteins.
• Antibody class: Mouse IgG2a
• Applications: This antibody can be used in ELISA and Western blotting.
• Presentation and storage: Purified antibody is available at a concentration of 1mg/ml in citrate-tris-Hcl buffer pH7.0, with 0.02%Proclin 300 as a preservative. The antibody may be stored at -20° C for one year in its original formulation. Additionally, antibody may be stored at 2° to 8° C for up to 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles of the diluted antibody.

Reviews

05/10/2021

Excellent after-sales service, solving many experimental problems.

12/30/2020

The GST protein I purchased is suitable for WB, and I have obtained satisfactory experimental results.

10/22/2020

The protein composition was stable and concentrated, allowing me to obtain satisfactory experimental results.

Q&As

Where is GST primarily located?

GST is mainly localized in the cytoplasm.

How does GST expression change under stress conditions?

Stress stimuli contributed to the up-regulation of GST gene expression, and GSTs showed higher enzymatic activities under different stress stimuli.

What is the function of GST?

GST plays an important role in phytoremediation, detoxification of xenobiotics, and oxidative stress metabolism.

What is the structure of the GST protein?

GST proteins are usually composed of homo- or heterodimers, and each subunit constituting a dimer contains two structural domains, the N-terminal and C-terminal domains.