Recombinant Human FCER1A cell lysate

• Cat.No.: FCER1A-1394HCL
• Product Overview: Human FCER1A / FcERI derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: FCER1A Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide [ Homo sapiens ]
• Official Symbol: FCER1A
• Synonyms: FCER1A; Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide; FCE1A; high affinity immunoglobulin epsilon receptor subunit alpha; Fc-epsilon RI-alpha; Fc epsilon RI alpha-chain; igE Fc receptor subunit alpha; Fc IgE receptor, alpha polypeptide; high affinity immunoglobulin epsilon receptor alpha-subunit; immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide; FcERI
• Gene ID: 2205
• mRNA Refseq: NM_002001
• Protein Refseq: NP_001992
• MIM: 147140
• UniProt ID: P12319
• Chromosome Location: 1q23
• Pathway: Asthma, organism-specific biosystem; Asthma, conserved biosystem; Fc epsilon RI signaling pathway, organism-specific biosystem; Fc epsilon RI signaling pathway, conserved biosystem; Fc-epsilon receptor I signaling in mast cells, organism-specific biosystem
• Function: IgE binding; receptor activity

Reviews

11/30/2022

FCER1A can maintain its biological activity and structural integrity under specific experimental conditions.

11/08/2022

FCER1A quality is stable and reliable, with no significant differences between batches.

01/15/2021

FCER1A has high chemical and physical stability and is suitable for long-term storage and use.

Q&As

Is FCER1A expression regulated?

Modulation of FCER1A expression can be achieved through techniques such as gene knockout, gene silencing, or gene transduction. In addition, its expression levels can also be affected by altering environmental factors such as nutrition, inflammation, etc.

How can I detect the expression level of FCER1A?

The expression level of FCER1A can be detected by gene expression profiling or immunohistochemistry.

Is there a role for FCER1A in the immune system?

FCER1A plays a key role in the immune system, recognizing and presenting antigens to T cell receptors, thereby initiating an adaptive immune response.

What diseases is FCER1A associated with?

Studies have shown that FCER1A is associated with the risk of some autoimmune diseases, inflammatory diseases, and certain cancers.

What is the significance of studying FCER1A?

Studying FCER1A can help to gain insight into the mechanisms of immune response and inflammatory response, which is of great significance for the development of new drugs and therapeutic strategies. In addition, it is also of great value for understanding the pathogenesis and prevention of diseases.

How is FCER1A related to other genes or proteins?

FCER1A has complex associations with other genes and proteins, such as its interaction with T cell receptors, inflammatory factors, etc.