Recombinant Human ADM cell lysate

• Cat.No.: ADM-1530HCL
• Product Overview: Human ADM / Adrenomedullin derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: ADM adrenomedullin [ Homo sapiens ]
• Official Symbol: ADM
• Synonyms: ADM; adrenomedullin; AM; preproadrenomedullin
• Gene ID: 133
• mRNA Refseq: NM_001124
• Protein Refseq: NP_001115
• MIM: 103275
• UniProt ID: P35318
• Chromosome Location: 11
• Pathway: Calcitonin-like ligand receptors, organism-specific biosystem; Class B/2 (Secretin family receptors), organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; HIF-1-alpha transcription factor network, organism-specific biosystem; Myometrial Relaxation and Contraction Pathways, organism-specific biosystem
• Function: adrenomedullin receptor binding; hormone activity; protein binding; receptor binding

Reviews

10/14/2020

The manufacturer has also provided me with technical support that is second to none, helping me troubleshoot any problems that I faced.

06/05/2020

I also appreciate the outstanding technical support offered by the manufacturer, which has saved me a lot of time and effort in troubleshooting my experiments.

05/11/2020

The manufacturer has earned my trust with their excellent technical support, which has been instrumental in simplifying my experiments and minimizing the margin of error.

12/15/2018

The protein I have decided on is of top-notch quality, showing high activity and reliability in my experiments.

Q&As

How are ADM proteins being studied for potential therapies for Alzheimer's disease?

ADM proteins have been studied for potential therapies for Alzheimer's disease because they promote the growth and survival of neurons. ADM has been shown to enhance cognitive function and reduce inflammation in animal models of Alzheimer's disease. ADM agonists, which mimic the activity of ADM, are being studied as potential therapies for Alzheimer's disease.

Can ADM proteins be used as a biomarker for cardiovascular disease?

ADM proteins have been studied as a potential biomarker for cardiovascular disease. High levels of ADM have been associated with an increased risk of cardiovascular disease, and measuring ADM levels in the blood may help identify patients at risk. However, further research is needed to determine the usefulness of ADM as a biomarker for cardiovascular disease.

How are ADM proteins measured in medical tests?

There are several methods for measuring ADM proteins in medical tests, including immunoassays, enzyme-linked immunosorbent assays (ELISAs), and radioimmunoassays (RIAs). These tests are typically performed on blood or urine samples. The levels of ADM proteins in these samples can be used to diagnose and monitor various medical conditions.

What are the functions of ADM proteins?

ADM proteins have multiple functions in the human body. They are involved in the regulation of blood pressure, cardiovascular function, angiogenesis, endothelial cell proliferation, and immune response. They also play a role in the development of various diseases, including cardiovascular disease, cancer, and inflammatory disorders.

What medical conditions are associated with abnormal ADM protein levels?

Abnormal levels of ADM proteins are associated with various medical conditions. Low levels of ADM proteins have been implicated in cardiovascular disease, sepsis, and inflammatory disorders. High levels of ADM proteins have been associated with pulmonary hypertension, kidney disease, and certain types of cancer.

Are ADM proteins targeted for medical treatment?

Yes, ADM proteins are a potential target for medical treatment. There are several drugs currently in development that target ADM activity, including ADM receptor antagonists and ADM-specific antibodies. These treatments may be useful for a variety of conditions, including cardiovascular disease, sepsis, and cancer.

How do ADM proteins interact with the immune system?

ADM proteins can interact with the immune system in several ways. ADM has been shown to have immunomodulatory effects, which means it can influence the activity of immune cells. ADM can inhibit the activity of inflammatory cells and decrease the production of pro-inflammatory cytokines. ADM has also been shown to have a chemotactic effect on immune cells, which means it can attract immune cells to sites of inflammation or infection.

Are there any lifestyle changes that can affect ADM protein levels?

There are several lifestyle changes that can affect ADM protein levels in the body. For example, exercise has been shown to increase ADM levels, while smoking has been shown to decrease ADM levels. Diet may also play a role in ADM protein levels, with some studies suggesting that a high-fat diet may decrease ADM levels. Patients should talk to their healthcare providers about lifestyle changes that may be beneficial for their specific conditions.

Do ADM protein levels change with age?

Yes, ADM protein levels tend to increase with age. This may be due to changes in cardiovascular function and endothelial cell function that occur with aging. Elevated ADM levels have been associated with age-related diseases such as hypertension and atherosclerosis.

How are ADM proteins being studied for potential cancer treatments?

ADM proteins are being studied for potential cancer treatments because they play a role in tumor growth and vascularization. ADM proteins promote angiogenesis, which is the process of forming new blood vessels, and can contribute to tumor growth and metastasis. Targeting ADM signaling may be a way to inhibit tumor growth and improve the effectiveness of current cancer therapies.

Can ADM proteins be used to treat sepsis?

ADM proteins have been studied as a potential treatment for sepsis, a life-threatening condition that occurs when the body's immune response to infection becomes dysregulated. ADM has anti-inflammatory and vasodilatory effects, which may help mitigate the effects of sepsis. ADM infusion has been shown to improve survival rates in animal models of sepsis, and clinical trials are ongoing to determine if ADM can be used as a treatment for sepsis in humans.

Are there any FDA-approved drugs that target ADM signaling?

There are currently no FDA-approved drugs that target ADM signaling. However, drugs that target ADM signaling are being studied in clinical trials for a variety of conditions, including sepsis, heart failure, and cancer. These drugs include ADM receptor agonists, which mimic the activity of ADM, and ADM-neutralizing antibodies, which block the activity of ADM.

Are there any risks associated with measuring ADM protein levels in medical tests?

There are generally no known risks associated with measuring ADM protein levels in medical tests. However, as with any medical test, there is a small risk of complications from the sample collection process, such as infection or bleeding. Patients should discuss any concerns they have about medical tests with their healthcare providers.

Can ADM proteins be used to predict mortality in critically ill patients?

ADM protein levels have been studied as a potential predictor of mortality in critically ill patients. High levels of ADM have been associated with an increased risk of death in patients with sepsis, heart failure, and other critical illnesses. Measuring ADM levels may help identify patients who are at high risk of mortality and may benefit from early interventions and aggressive treatment.