Recombinant Human CD5 cell lysate

• Cat.No.: CD5-2572HCL
• Product Overview: Human CD5 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: CD5 CD5 molecule [ Homo sapiens ]
• Official Symbol: CD5
• Synonyms: CD5; CD5 molecule; CD5 antigen (p56 62) , LEU1; T-cell surface glycoprotein CD5; T1; CD5 antigen (p56-62); lymphocyte antigen T1/Leu-1; LEU1
• Gene ID: 921
• mRNA Refseq: NM_014207
• Protein Refseq: NP_055022
• MIM: 153340
• UniProt ID: P06127
• Chromosome Location: 11q13
• Pathway: B Cell Receptor Signaling Pathway, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; T Cell Receptor Signaling Pathway, organism-specific biosystem
• Function: glycoprotein binding; protein binding; receptor activity; scavenger receptor activity; transmembrane signaling receptor activity

Reviews

08/18/2022

The stability of CD5 is very good, and even if it is stored for a long time, it will not affect its performance.

12/20/2021

I have recommended it to other students, the price is affordable, the operation is convenient, and the effect is good.

11/28/2020

I'm very happy with the results, the stability is strong.

Q&As

What is the interaction between CD5 and other biomolecules?

There are interactions between CD5 and other biomolecules. For example, it binds to antigens and antibodies and participates in the activation and differentiation of immune cells, and also binds to other signal transduction molecules and participates in cell signal transduction.

Which biomolecules can be used as molecular markers for CD5?

Certain biomolecules can be used as molecular markers of CD5, including CD5 antigens detected on the surface of lymphocytes, as well as related proteins and genes that interact with CD5. These markers can be used for early diagnosis of diseases, prognosis judgment, or monitoring the effectiveness of treatment.

How can the efficacy and safety of treatments for CD5 be assessed?

Assessing the efficacy and safety of treatments targeting CD5 requires clinical trials and drug monitoring. The effectiveness of the treatment was evaluated by designing a rigorous clinical trial protocol that compared the disease remission rate, symptom improvement, and quality of life improvement between the treatment and control groups. At the same time, drug monitoring is used to monitor the safety and efficacy of drugs in actual use, adjust treatment regimens in a timely manner, maximize the efficacy of drugs and reduce the risk of side effects.

What is the structure of CD5?

CD5 has a typical immunoglobulin superfamily domain, which is a single-stranded transmembrane glycoprotein that is distributed in an asymmetrical manner across the cell membrane. This structure allows it to bind to antigens and antibodies and participate in the immune response.

What diseases are CD5 associated with?

CD5 has been linked to a number of immune system-related diseases. For example, certain autoimmune diseases, allergic reactions, transplant rejection, etc. In addition, CD5 has been implicated in the development and progression of certain tumors.

How is the therapeutic strategy of CD5 evaluated?

Treatment strategies for CD5 include inhibition of its activity, regulation of its expression, and gene therapy. For example, the development of antibody drugs or small molecule inhibitors against CD5 to inhibit its overactivation in the immune response, or the regulation of CD5 expression through gene knockout or overexpression techniques.