Recombinant Human TPT1 293 Cell Lysate

• Cat.No.: TPT1-831HCL

Specification

• Description: Antigen standard for tumor protein, translationally-controlled 1 (TPT1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: TPT1 tumor protein, translationally-controlled 1 [ Homo sapiens ]
• Official Symbol: TPT1
• Synonyms: TPT1; tumor protein, translationally-controlled 1; translationally-controlled tumor protein; fortilin; TCTP; p23; histamine-releasing factor; HRF; p02; FLJ27337
• Gene ID: 7178
• mRNA Refseq: NM_003295
• Protein Refseq: NP_003286
• MIM: 600763
• UniProt ID: P13693
• Chromosome Location: 13q14
• Pathway: PLK1 signaling events, organism-specific biosystem

Reviews

03/16/2022

The quality is very good, the experimental effect is as expected, will continue to use.

06/27/2021

The product has reliable and stable performance, and is suitable for scientific research.

08/28/2020

We are reassured that their TPT1 quality is consistent.

Q&As

What is the association between TPT1 and tumorigenesis?

It is highly expressed in a variety of tumors, which is related to the occurrence and progression of tumors. Studies have shown that TPT1 may be involved in processes such as cell cycle regulation, apoptosis, and DNA repair.

How does TPT1 interact with other molecules or drugs?

There are multiple interactions between TPT1 and other molecules and drugs. For example, TPT1 can bind to certain signaling molecules, which can affect the growth and differentiation of cells. In addition, some drugs can also affect TPT1 expression levels or function.

What are the treatments for TPT1?

There is currently no specific treatment for TPT1. However, drugs that target their associated signal transduction pathways or binding targets may have potential therapeutic value.

Can TPT1 expression levels be used as a biomarker for tumor diagnosis or prognosis?

At present, the expression level of TPT1 as a biomarker for tumor diagnosis or prognosis is not sufficient, and further validation is needed.

How can I detect TPT1 expression levels?

TPT1 expression levels can be detected by gene sequencing, immunohistochemistry, and western blotting.

Are there have possible aspects of future research on TPT1?

Future research on TPT1 may involve more in-depth molecular mechanism research, new drug development, and TPT1-based targeted therapy strategies. In addition, research may also address the function and mechanism of action of TPT1 in other tissues and organs.