Recombinant Human PHB 293 Cell Lysate

• Cat.No.: PHB-3241HCL

Specification

• Description: Antigen standard for prohibitin (PHB) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PHB prohibitin [ Homo sapiens ]
• Official Symbol: PHB
• Synonyms: PHB; prohibitin; PHB1
• Gene ID: 5245
• mRNA Refseq: NM_002634
• Protein Refseq: NP_002625
• MIM: 176705
• UniProt ID: P35232
• Chromosome Location: 17q21
• Function: histone deacetylase binding; protein binding; transcription regulatory region DNA binding

Reviews

08/12/2023

The catalytic activity is amazing, and the mild nature of the mild nature is appreciated for the highly efficient catalysis achieved under mild conditions.

06/16/2023

We like the purity and precision of their products, and the results are accurate and reliable.

07/03/2021

Their protein production process is strictly controlled, and the quality of products is guaranteed.

Q&As

What is the role of PHB proteins in cell signaling?

PHB proteins play an important role in cell signal transduction. It can act as a regulator of signal transduction pathways, influencing processes such as cell proliferation, differentiation, and apoptosis. The specific mechanism of action needs to be further studied.

How can I detect the expression level of PHB protein?

The expression level of PHB protein can be detected by immunohistochemistry, western blotting, and real-time PCR. These techniques can detect protein or mRNA levels of PHB proteins in tissues or cells to assess changes in their expression in disease.

Where is PHB protein located in cells?

PHB proteins are mainly localized in the inner mitochondrial membrane and nucleus. It plays a role in regulating mitochondrial function and structure on the inner mitochondrial membrane and is involved in processes such as gene transcription and DNA damage repair in the nucleus.

What are the biological functions of PHB proteins?

PHB proteins have a variety of biological functions. It is involved in mitochondrial biogenesis and regulates mitochondrial number and function, participates in the process of cell proliferation and apoptosis, affects cell growth and death, and also participates in nuclear activities such as gene transcription and DNA damage repair.

What are the therapeutic strategies for PHB protein?

Therapeutic strategies for PHB proteins include inhibition of its activity, regulation of its expression, and gene therapy. For example, the development of small molecule inhibitors or antibody drugs against PHB proteins to inhibit their aberrant activity in diseases, or the regulation of PHB protein expression through gene knockout or overexpression techniques.

Are interactions between PHB proteins and other proteins present?

There is an interaction between PHB protein and other proteins. It can interact with mitochondria-related proteins to regulate mitochondrial function and structure, and can also interact with nucleus-related proteins to participate in processes such as gene transcription and DNA damage repair.