Recombinant Human PAEP 293 Cell Lysate

• Cat.No.: PAEP-3469HCL

Specification

• Description: Antigen standard for progestagen-associated endometrial protein (PAEP), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PAEP progestagen-associated endometrial protein [ Homo sapiens ]
• Official Symbol: PAEP
• Synonyms: PAEP; progestagen-associated endometrial protein; glycodelin; alpha uterine protein; GD; GdA; GdF; GdS; glycodelin A; glycodelin F; glycodelin S; MGC138509; MGC142288; PAEG; PEP; PP14; PP14 protein (placental protein 14); pregnancy associated endometrial alpha 2 globulin; progesterone associated endometrial protein; PEG; glycodelin-A; glycodelin-F; glycodelin-S; placental protein 14; progesterone-associated endometrial protein; pregnancy-associated endometrial alpha-2 globulin; pregnancy-associated endometrial alpha-2-globulin; progestagen-associated endometrial protein (placental protein 14, pregnancy-associated endometrial a
• Gene ID: 5047
• mRNA Refseq: NM_001018049
• Protein Refseq: NP_001018059
• MIM: 173310
• UniProt ID: P09466
• Chromosome Location: 9q34
• Function: binding; transporter activity

Reviews

08/11/2020

With the manufacturer's outstanding technical support, you can be assured that any challenges you encounter will be efficiently addressed.

05/04/2020

Its superior quality, combined with the manufacturer's exceptional technical support, provides researchers with the confidence they need to conduct successful experiments and obtain meaningful results.

01/02/2019

Researchers who have utilized the PAEP protein laud its reliability and effectiveness in their studies.

Q&As

Are there any limitations or challenges associated with using PAEP protein in clinical settings?

Challenges may include variations in individual PAEP levels and the need for standardized assays for accurate measurements.

Can PAEP protein be used as a marker for recurrent pregnancy loss?

Some studies suggest a potential association between low PAEP levels and recurrent pregnancy loss, but more research is needed to establish a definitive link.

How does PAEP protein contribute to the understanding of endometrial function?

PAEP's expression and regulation in the endometrium provide insights into the complex processes involved in implantation and endometrial function.

How is PAEP protein utilized in monitoring high-risk pregnancies?

Elevated or reduced PAEP levels may indicate a risk of complications during pregnancy, such as preeclampsia, allowing for early intervention and management.

Can PAEP protein be a target for therapeutic interventions in reproductive medicine?

Research suggests that manipulating PAEP levels may have therapeutic potential in addressing certain reproductive disorders, though further studies are needed.