Recombinant Human MAPK14(M1-S360) was expressed in E. coli.
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 20% glycerol
Specific kinase activity (Pi transfer): 893 pmol/µg×minATP-KM: 2.7 µm
GAFQSIIHAKRT YRELRLLKHM KHENVIGLLD VFTPARSLEE FNDVYLVTHL MGADLNNIVK MSQERPTF YRQELNKTIW EVPERYQNLS PVGSGAYGSV CAAFDTKTGL RVAVKKLSRPCQKLTDDHVQ FLIYQILRGL KYIHSADIIH RDLKPSNLAV NEDCELKILD FGLARHTDDE MTGYVATRWY RAPEIMLNWM HYNQTVDIWS VGCIMAELLT GRTLFPGTDH INQLQQIMRLTGTPPAYLIN RMPSHEARNY IQSLTQMPKM NFANVFIGAN PLAVDLLEKM LVLDSDKRIT AAQALAHAYF AQYHDPDDEP VADPYDQSFE SRDLLIDEWK SLTYDEVISF VPPPLDQEEMES
Storage temperature: -80 centigrade.For complete recovery, mix well and spin before use. Product must not be stored in diluted solutions, aliquots below 10µl are not advisable. Avoid repeated freeze-thaw cycles!