Recombinant Human protein kinase C, Alpha, His-tagged
Cat.No. : | PRKCA-58H |
Product Overview : | Recombinant Human Protein Kinase C alpha produced inSf9is a glycosylated, polypeptide chain containing amino acids 2-672 and having a molecular mass of 80 KD. This protein is the full-length form of the protein with an amino terminal poly His-tag. PKC- is purified by proprietary chromatographic techniques. |
- Specification
- Gene Information
- Related Products
Cat. No. : | PRKCA-58H |
Description : | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. |
Source : | Sf9 insect cells. |
Unit Definition : | TheUnit Definition is 7200-9000 units/mg. One unit of PKC-activity transfers 1 nmole of phosphate to a PKC substrate peptide (myelin basic protein 4-14) per minute at 30°C. Recombinant human active PKC-also phosphorylates full length myelin basic protein, histone, MARKS, and GSK-3. Activity requires incubation with lipid activators, and may vary depending on the substrate and reaction conditions. |
Purity : | Greater than 95% as determined by SDS-PAGE. |
Formulation : | PKC- is supplied at a of 0.3mg/ml in 50mM Tris, pH 7.4, 0.1M NaCl, 20% glycerol, 1mM DTT, 0.1mM EDTA, 0.2mM PMSF and 0.03% Brij-35. |
Storage : | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
Gene Name : | PRKCA protein kinase C, alpha [ Homo sapiens ] |
Synonyms : | PRKCA; protein kinase C, alpha; AAG6; PKCA; PRKACA; MGC129900; MGC129901; PKC-alpha;EC2.7.11. 13;Protein kinase C alpha type; aging-associated gene 6; protein kinase C alpha type |
Gene ID : | 5578 |
mRNA Refseq : | NM_002737 |
Protein Refseq : | NP_002728 |
UniProt ID : | P17252 |
MIM : | 176960 |
Chromosome Location : | 17q22-q24 |
Pathway : | Calcium signaling pathway; ErbB signaling pathway; Fc epsilon RI signaling pathway; Fc gamma R-mediated phagocytosis; Focal adhesion; Glioma; GnRH signaling pathway; Leukocyte transendothelial migration; Long-term potentiation; Long-term depression; MAPK signaling pathway; Melanogenesis; Natural killer cell mediated cytotoxicity; Non-small cell lung cancer; Pathogenic Escherichia coli infection - EHEC; Pathways in cancer; Phosphatidylinositol signaling system; Tight junction; VEGF signaling pathway; Vascular smooth muscle contraction; Vibrio cholerae infection; Wnt signaling pathway; Diabetes pathways; Hemostasis; Synaptic Transmission |
Function : | ATP binding; calcium ion binding; calcium-dependent protein kinase C activity; nucleotide binding; protein binding; transferase activity; zinc ion binding |
Products Types
◆ Recombinant Protein | ||
PRKCA-011H | Recombinant Human PRKCA Protein, GST-tagged | +Inquiry |
PRKCA-0187H | Recombinant Human PRKCA Protein (A2-V672), Tag Free | +Inquiry |
PRKCA-7092M | Recombinant Mouse PRKCA Protein, His (Fc)-Avi-tagged | +Inquiry |
PRKCA-3422R | Recombinant Rhesus Macaque PRKCA Protein, His (Fc)-Avi-tagged | +Inquiry |
PRKCA-3909H | Recombinant Human PRKCA Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
PRKCA-499HCL | Recombinant Human PRKCA lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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