Recombinant Human INHA 293 Cell Lysate

• Cat.No.: INHA-5204HCL

Specification

• Description: Antigen standard for inhibin, alpha (INHA) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: INHA inhibin, alpha [ Homo sapiens ]
• Official Symbol: INHA
• Synonyms: INHA; inhibin, alpha; inhibin alpha chain; A-inhibin subunit
• Gene ID: 3623
• mRNA Refseq: NM_002191
• Protein Refseq: NP_002182
• MIM: 147380
• UniProt ID: P05111
• Chromosome Location: 2q33-qter
• Pathway: Glycoprotein hormones, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Ovarian Infertility Genes, organism-specific biosystem; Peptide hormone biosynthesis, organism-specific biosystem
• Function: cytokine activity; growth factor activity; hormone activity; inhibin beta-A binding; protein binding; contributes_to protein binding; protein heterodimerization activity; receptor binding

Reviews

04/29/2022

The product’s stability over extended periods was a significant advantage for our long-term research projects.

04/16/2020

Consistently achieving specific outcomes validated the pivotal role of the product in our research studies.

09/11/2017

The responsive customer support team provided quick resolutions to any queries or issues we encountered.

Q&As

Does INHA have implications in the context of cancer research?

INHA's role in cancer research requires further investigation to determine its potential involvement in tumorigenesis.

What roles does INHA protein play in cellular processes?

INHA protein serves various roles in cellular processes, potentially affecting cell growth, signaling, and development.

What is known about the expression patterns of INHA in different tissues?

INHA may exhibit tissue-specific expression patterns, and its significance in various tissues is a subject of ongoing study.

Can you describe the specific functions of INHA in gene regulation?

INHA may have functions related to gene regulation, influencing transcription and other cellular processes.

How does INHA contribute to cellular pathways and signaling?

INHA likely contributes to cellular pathways and signaling cascades, impacting various cellular functions.

Are there any known interactions between INHA and other proteins or molecules?

INHA may interact with other proteins or molecules, potentially affecting its cellular functions.

Have genetic mutations or alterations been linked to INHA?

While INHA mutations or alterations are not extensively studied, they could be relevant to cellular processes and warrant further exploration.