Recombinant Human MMP7
Cat.No. : | MMP7-40H |
Product Overview : | Recombinant Human Matrix Metalloproteinase-7 produced inE.Coliis a single, non-glycosylated, polypeptide chain containing having a molecular mass of 19.13 kDa. The MMP-7 is purified by proprietary chromatographic techniques. |
- Specification
- Gene Information
- Related Products
Cat. No. : | MMP7-40H |
Description : | Matrix metalloproteinase-7 (MMP-7) also known as matrilysin and PUMP (EC 3.4.24.23) cleaves a number of substrates including collagen types IV and X, elastin, fibronectin, gelatin, laminin and proteoglycans. MMP-7 is closely related to the stromelysin family members but is encoded by a different gene. MMP-7 is the smallest of all the MMPs consisting of a pro-peptide domain and a catalytic domain. It lacks the hemopexin-like domain common to other members of the MMPs. MMP-7 is secreted as a 28 kDa proenzyme and can be activated in vitro by organomercurials and trypsin and in vivo by MMP-3 to a 18 kDa active MMP-7 enzyme. Once activated, MMP-7 can activate pro-MMP-1 and pro-MMP-9 but not pro-MMP-2. MMP-7 is widely expressed having been reported in elevated levels in cycling endometrium as well as in colorectal cancers and adenomas, hepatocellular carcinomas, rectal carcinomas, and approximately 50% of gliomas. |
Source : | Escherichia Coli. |
Physical Appearance : | Sterile clear liquid solution. |
Purity : | Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Formulation : | The protein (0.83 mg/ml) contains the following additives 10 mM HEPES (pH 7.4), 5 mM CaCl2 and 150 mM NaCl. |
Biological Activity : | The specific activity was found to be 3100 IU/mg. |
Unit Definition : | One unit is defined as the digestion of 1 µg Azocoll/min at 37°C. |
Recommended Use : | Western Blotting (1:1K-5K for neat serum and 1-10 ug/ml for affinity pure antibody using ECL technique). ELISA: Control peptide can be used to coat ELISA plates at 1 ug/ml and detected with antibodies (1:10-50K for neat serum and 0.5-1 ug/ml for affinity pure). Histochemistry & Immunofluorescence: Not tested. |
Stability : | Matrilysin although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles. |
Gene Name : | MMP7 matrix metallopeptidase 7 (matrilysin, uterine) [ Homo sapiens ] |
Synonyms : | MMP7; matrix metallopeptidase 7 (matrilysin, uterine); MMP-7; MPSL1; PUMP-1; matrix metalloproteinase 7; matrin; uterine matrilysin; EC 3.4.24.23; Matrix metalloproteinase-7; Pump-1 protease; Uterine metalloproteinase; PUMP1; Matrin |
Gene ID : | 4316 |
mRNA Refseq : | NM_002423 |
Protein Refseq : | NP_002414 |
MIM : | 178990 |
UniProt ID : | P09237 |
Chromosome Location : | 11q21-q22 |
Pathway : | Wnt signaling pathway |
Function : | calcium ion binding; metalloendopeptidase activity; peptidase activity; zinc ion binding |
Products Types
◆ Recombinant Protein | ||
Mmp7-10595M | Recombinant Mouse Mmp7 Protein, His (Fc)-Avi-tagged | +Inquiry |
MMP7-5435H | Recombinant Human MMP7 Protein, GST-tagged | +Inquiry |
MMP7-50H | Active Recombinant Human MMP7 Protein, TG-8H-GGQ-tagged | +Inquiry |
MMP7-1458R | Recombinant Rat MMP7 Protein (98-267 aa), His-tagged | +Inquiry |
MMP7-001H | Recombinant Human MMP7 Protein, His-tagged | +Inquiry |
◆ Native Protein | ||
MMP7-28205TH | Native Human MMP7 | +Inquiry |
◆ Lysates | ||
MMP7-2883HCL | Recombinant Human MMP7 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
0
Inquiry Basket