Recombinant Human DHFR cell lysate

• Cat.No.: DHFR-353HCL

Specification

• Species: Hamster
• Tissue Type: Ovary
• Preparation method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 2 mg/ml.
• Size: 200 ug
• Recommended Usage: Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Storage Buffer: In modified RIPA Lysis Buffer.
• Applications: Western Blot;Immunoprecipitation

Gene Information

• Gene Name: DHFR dihydrofolate reductase [ Homo sapiens ]
• Official Symbol: DHFR
• Synonyms: DYR; DHFRP1
• Gene ID: 1719
• mRNA Refseq: NM_000791
• Protein Refseq: NP_000782
• MIM: 126060
• UniProt ID: P00374
• Chromosome Location: 5q11.2-q13.2
• Pathway: Cell Cycle, organism-specific biosystem; Defective AMN causes hereditary megaloblastic anemia 1, organism-specific biosystem; Defective GIF causes intrinsic factor deficiency, organism-specific biosystem

Reviews

04/16/2023

The DHFR protein offered by the manufacturer is of exceptional quality, making it an ideal choice to fulfill my experimental needs.

03/20/2017

Another crucial aspect where the manufacturer excels is their efficient supply chain management.

08/31/2016

The technical support provided by the manufacturer is invaluable in overcoming experimental hurdles and optimizing the utilization of DHFR protein.

Q&As

Can you name a common DHFR inhibitor drug used in cancer treatment?

Methotrexate is a common DHFR inhibitor drug used in cancer treatment.

Are there ongoing research efforts to develop new DHFR inhibitors for clinical use?

Yes, scientists continue to research and develop new DHFR inhibitors with improved efficacy and reduced side effects for various clinical applications.

Are there any drug-resistant strains that have developed due to DHFR inhibitors?

Yes, drug-resistant strains have emerged due to mutations in DHFR genes, reducing the effectiveness of DHFR inhibitor drugs.

Are there any side effects associated with DHFR inhibitor drugs like Methotrexate?

Yes, common side effects of DHFR inhibitors include nausea, vomiting, decreased blood cell counts, and liver toxicity.

Can you name an antibiotic that inhibits DHFR in bacteria?

Trimethoprim is an antibiotic that inhibits DHFR in bacteria.