Active Recombinant Human NEK9, GST-tagged

• Cat.No.: NEK9-1064H
• Product Overview: Recombinant human NEK9 (1-979; deleted fragment of 346-731) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: NEK9 is a member of the NEK family and has high homology to NEK1, NEK3 and NEK4. NEK9 is activated during mitosis and binds specifically to RAN GTPase and is a substrate for CDC2 phosphorylation. NEK9 plays a role in the control of mitotic progression and is regulated by CDC2 and RAN GTPase. Overexpression of both active and inactive variants of NEK9 is toxic to cells and inhibits cell division causing abnormal nuclear morphologies. NEK9 can catalyze the phosphorylation of recombinant NEK6 and NEK7 in vitro leading to its activation. This suggests that NEK9 may be responsible for activation of NEK6 and NEK7 during mitosis.
• Source: Sf9 insect cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 120 nmol/min/mg
• Molecular Mass: ~115 kDa
• Protein length: 1-979; deleted fragment of 346-731
• Purity: >75%
• Applications: Kinase Assay; Western Blot
• Stability: 1 yr at -70oC from date of shipment.
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 ug/ul

Gene Information

• Gene Name: NEK9 NIMA (never in mitosis gene a)- related kinase 9 [ Homo sapiens ]
• Official Symbol: NEK9
• Synonyms: NEK9; NIMA (never in mitosis gene a)- related kinase 9; serine/threonine-protein kinase Nek9; DKFZp434D0935; MGC16714; Nek8; NERCC; nercc1 kinase; nimA-related kinase 8; NIMA-related kinase Nek8; nimA-related protein kinase 9; never in mitosis A-related kinase 9; NERCC1; MGC138306
• Gene ID: 91754
• mRNA Refseq: NM_033116
• Protein Refseq: NP_149107
• MIM: 609798
• UniProt ID: Q8TD19
• Chromosome Location: 14q24.2
• Pathway: Activation of NIMA Kinases NEK9, NEK6, NEK7; Mitotic Prophase; Nuclear Pore Complex (NPC) Disassembly
• Function: ATP binding; metal ion binding; protein kinase binding; protein serine/threonine kinase activity