Active Recombinant Human RBP4 Protein (Glu19-Leu201), C-6×His tagged

• Cat.No.: RBP4-354H
• Product Overview: Recombinant Human RBP4 Protein (Glu19-Leu201) with a C-terminal Ile and 6-His tag was expressed in Mouse myeloma cell line.

Specification

• Description: This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells.
• Source: Mouse myeloma cell line
• Species: Human
• Tag: C-6×His
• Bio-activity: Measured by its ability to bind all-trans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4. >1.0 μM all-trans retinoic acid is bound under the described conditions.
• Molecular Mass: Predicted Molecular Mass: 22 kDa
  SDS-PAGE: 22 kDa, reducing conditions
• Protein Length: Glu19-Leu201
• N-terminal Sequence Analysis: Glu19
• Endotoxin: <1.0 EU/μg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
• Reconstitution: Reconstitute at 500 μg/mL in sterile 50 mM Tris, 10 mM CaCl2 and 150 mM NaCl, pH 7.5.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: RBP4 retinol binding protein 4, plasma [ Homo sapiens (human) ]
• Official Symbol: RBP4
• Synonyms: RBP4; retinol binding protein 4, plasma; retinol-binding protein 4; RBP; PRBP; plasma retinol-binding protein; retinol-binding protein 4, interstitial
• Gene ID: 5950
• mRNA Refseq: NM_006744
• Protein Refseq: NP_006735
• MIM: 180250
• UniProt ID: P02753