Recombinant Human RGMB protein, His-tagged
Recombinant Human RGMB(Gly87-Asp209 (N-terminus chain) & Pro210-Ser452 (C-terminus chain)) fused with His tag at C-terminal was expressed in NS0.
Predicted N Terminal:
Gly87 & Pro210
Lyophilized from a 0.2 μm filtered solution in PBS.
Measured by its binding ability in a functional ELISA. Immoblized rhBMP-4 at 1 µg/mL (100 µL/well) can bind rhRGM-B with a linear range of 10-500 ng/mL.
Predicted Molecular Mass: 13.4 (N-terminus chain), 27.7 (C-terminus chain) kDa;SDS-PAGE: 20 kDa and 35 kDa, reducing conditions.
Gly87-Asp209 (N-terminus chain) & Pro210-Ser452 (C-terminus chain)
<0.01 EU per 1 μg of the protein by the LAL method.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 100 μg/mL in sterile PBS.