Active Recombinant Human EPCAM protein, Fc-tagged

• Cat.No.: EPCAM-475H
• Product Overview: Recombinant Human EPCAM(Gln24 - Lys265) fused with Fc tag at C-terminal was expressed in NS0.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc
• Predicted N Terminal: Gln24
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line.When 5 x 10^4 cells/well are added to recombinant human EpCAM/Fc Chimera and human Fibronectin (0.5 μg/mL) coated plates, cell adhesion is enhanced in a dose dependent manner after 45 minutes at 37 centigrade. The ED50 for this effect is typically 0.7-2.8 μg/mL. Measured by its binding ability in a functional ELISA. The activity of this protein is measured in a homophilic binding assay.
• Molecular Mass: Predicted Molecular Mass: 54 (monomer) kDa;SDS-PAGE: 60-70 kDa, reducing conditions
• Protein length: Gln24 - Lys265
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: EPCAM epithelial cell adhesion molecule [ Homo sapiens ]
• Official Symbol: EPCAM
• Synonyms: EPCAM; epithelial cell adhesion molecule; antigen identified by monoclonal AUA1 , M4S1, MIC18, TACSTD1, tumor associated calcium signal transducer 1; 17 1A; 323/A3; CD326; CO 17A; EGP 2; EGP34; EGP40; Ep CAM; ESA; GA733 2; HEA125; KS1/4; KSA; Ly74; MH99; MK 1; MOC31; TACST 1; TROP1; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated antigen; tumor-associated calcium signal transducer 1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); M4S1; MK-1; DIAR5; EGP-2; MIC18; EGP314; HNPCC8; TACSTD1; GA733-2
• Gene ID: 4072
• mRNA Refseq: NM_002354
• Protein Refseq: NP_002345
• MIM: 185535
• UniProt ID: P16422
• Chromosome Location: 2p21
• Function: protein binding

Reviews

02/13/2022

Reliable for assays, satisfied customer.

09/09/2019

High-quality product, consistent results.

04/03/2019

Quick delivery, product as described.

Q&As

In which types of tissues is EpCAM predominantly expressed?

EpCAM is highly expressed in epithelial tissues and some cancer cells.

How does EpCAM contribute to cell adhesion?

It has a controversial role in cell adhesion, possibly contributing to both cell-cell adhesion and anti-adhesive properties.

What is the primary function of EpCAM in cells?

EpCAM primarily functions in cell signaling, proliferation, and migration.

What role does EpCAM play in cancer progression?

It often has a pro-tumorigenic role, promoting cancer cell proliferation, migration, and invasion.

How is EpCAM used as a biomarker in cancer diagnosis?

EpCAM is used as a biomarker to detect and isolate circulating tumor cells.

What therapeutic strategies are being explored involving EpCAM?

Strategies include developing EpCAM-targeted therapies for cancer treatment.

Are there known genetic alterations in EpCAM associated with specific health conditions?

Genetic alterations in EpCAM are linked to some hereditary cancer syndromes.