Recombinant Human CDH5(Asp48-Gln599) fused with His tag at C-terminal was expressed in HEK293.
Predicted N Terminal:
Lyophilized from a 0.2 μm filtered solution in PBS.
Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. The ED50 for this effect is typically 20-100 ng/mL.Optimal dilutions should be determined by each laboratory for each application.
Predicted Molecular Mass: 63 kDa;SDS-PAGE: 80-94 kDa, reducing conditions
<0.10 EU per 1 mug of the protein by the LAL method.
>95%, by SDS-PAGE with silver staining
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 250 μg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.