Active Recombinant Human SOD1

• Cat.No.: SOD1-1432H
• Product Overview: Recombinant Human Superoxide Dismutase produced in E. coli. is a stable dimer of two identical subunits, non-glycosylated, containing 308 amino acid residues, two pairs of disulfide bonds and having a combined molecular mass of 31.6kDa.

Specification

• Description: Cu/Zn Human Superoxide Dismutase is a stable dimer of identical subunits with a combined molecular mass of 31.6kD. This enzyme dismutes the superoxide radical to molecular oxygen. This enzyme has been expressed in E. coli and purified using sequential chromatography steps.
• Source: E. coli
• Species: Human
• Form: Lyophilized from a 0.2μm filtered solution in 50mM Phosphate buffer, pH7.4.
• Bio-activity: ≥7000U/mg
• Endotoxin: ≤1EU/μg, determined by the LAL method.
• Purity: ≥95%, as determined by reduced SDS-PAGE Dimer ≥90%, as determined by SEC-HPLC.
• Usage: FOR RESEARCH ONLY
• Storage: Lyophilized samples are stable for greater than six months from date of receipt at -20 centigrade to -70 centigrade. The reconstituted samples can be stored under sterile conditions at 2-8 centigrade for one month or at -20 centigrade to -70 centigrade for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized rHuSOD in sterile ddH2O.

Gene Information

• Gene Name: SOD1 superoxide dismutase 1, soluble [ Homo sapiens ]
• Official Symbol: SOD1
• Synonyms: ALS; SOD; ALS1; IPOA; hSod1; HEL-S-44; homodimer; superoxide dismutase [Cu-Zn]; Cu/Zn superoxide dismutase; SOD, soluble;epididymis secretory protein Li 44; indophenoloxidase A; superoxide dismutase, cystolic
• Gene ID: 6647
• mRNA Refseq: NM_000454
• Protein Refseq: NP_000445
• MIM: 147450
• UniProt ID: P00441
• Chromosome Location: 21q22.11
• Pathway: AGE/RAGE pathway, organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Detoxification of Reactive Oxygen Species, organism-specific biosystem
• Function: Rac GTPase binding; chaperone binding; copper ion binding