Active Recombinant Human SPHK1 Protein, Met & His-tagged

• Cat.No.: SPHK1-741H
• Product Overview: Recombinant Human SPHK1 (Accession # NP_068807) Asp2-Leu398, fused with an N-terminal Met and 6-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Source: Baculovirus Sf21 Insect Cells
• Species: Human
• Tag: & His
• Predicted N Terminal: Inconclusive result, Met predicted. Protein identity confirmed by MS analysis of tryptic fragments.
• Form: Supplied as a 0.2 µm filtered solution in MES, NaCl, Glycerol and DTT.
• Bio-activity: Measured by its ability to phosphorylate Omega-(7-nitro-2-1,3-benzoxadiazol-4-yl)-D-erythro-sphingosine (NBD-sphingosine).The specific activity is >200 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human SPHK1, Met & His-tagged has a calculated MW of 45 kDa. In SDS-PAGE migrates as 40-43 kDa, reducing conditions.
• Protein length: Asp2-Leu398
• Purity: >75%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: SPHK1 sphingosine kinase 1 [ Homo sapiens ]
• Official Symbol: SPHK1
• Synonyms: SPHK1; sphingosine kinase 1; SPHK; SK 1; SPK 1
• Gene ID: 8877
• mRNA Refseq: NM_001142601
• Protein Refseq: NP_001136073
• MIM: 603730
• UniProt ID: Q9NYA1
• Chromosome Location: 17q25.2