Active Recombinant Human TPST2 Protein, His-tagged

• Cat.No.: TPST2-1006H
• Product Overview: Recombinant Human TPST2 (Accession # O60704) Gln26-Ser377, fused with an N-terminal CD33 signal sequence and a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Human
• Tag: His
• Predicted N Terminal: No N-terminal sequence was revealed by sequencing. The Gln residue maybe blocked. Protein identity confirmed by MS analysis of tryptic fragments.
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to transfer sulfate from PAPS to PSGL-1 peptide (Gln-Ala-Thr-Glu-Tyr-Glu-Tyr-Leu-Asp-Tyr-Asp-Phe-Leu-Pro-Glu-Thr)The specific activity is >20 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human TPST2, His-tagged has a calculated MW of 40 kDa. In SDS-PAGE migrates as 40-50 kDa, reducing conditions.
• Protein length: Gln26-Ser377
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile 4 mM HCI containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: TPST2 tyrosylprotein sulfotransferase 2 [ Homo sapiens ]
• Official Symbol: TPST2
• Synonyms: TPST2; tyrosylprotein sulfotransferase 2; protein-tyrosine sulfotransferase 2; TPST-2; tyrosylprotein sulfotransferase-2; tyrosylprotein phosphotransferase 2
• Gene ID: 8459
• mRNA Refseq: NM_001008566
• Protein Refseq: NP_001008566
• MIM: 603126
• UniProt ID: O60704
• Chromosome Location: 22q12.1