Active Recombinant Human PGK1 Protein, His-tagged

• Cat.No.: PGK1-1102H
• Product Overview: Recombinant Human PGK1 (Accession # P00558) Met1-Ile417, fused with an C-terminal 10-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Source: Baculovirus Sf21 Insect Cells
• Species: Human
• Tag: His
• Predicted N Terminal: No results obtained
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol.
• Bio-activity: Measured by NADH production in a reaction coupled with GAPDH.The specific activity, as measured under the described condtions, is >50,000 pmol/min/µg.
• Molecular Mass: Recombinant Human PGK1, His-tagged has a calculated MW of 46 kDa. In SDS-PAGE migrates as 42 kDa, reducing conditions.
• Protein length: Met1-Ile417
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: PGK1 phosphoglycerate kinase 1 [ Homo sapiens ]
• Official Symbol: PGK1
• Synonyms: PGK1; phosphoglycerate kinase 1; PRP 2; primer recognition protein 2; cell migration-inducing gene 10 protein; PGKA; MIG10; MGC8947; MGC117307; MGC142128
• Gene ID: 5230
• mRNA Refseq: NM_000291
• Protein Refseq: NP_000282
• MIM: 311800
• UniProt ID: P00558
• Chromosome Location: Xq13.3

Reviews

03/14/2020

manufacturers may offer customization options, allowing researchers to tailor the PGK1 protein to their specific needs.

02/27/2020

This may include options like different formulations, protein modifications, or tagging techniques. Customization can enhance the versatility of the protein and optimize its utility in the intended research.

04/15/2016

They can also address any questions or concerns regarding the PGK1 protein, thereby facilitating smooth and efficient research execution.

Q&As

How is PGK1 linked to neurological disorders?

PGK1 dysfunction has been associated with neurological conditions like Alzheimer's disease and Parkinson's disease due to its impact on cellular energy metabolism.

In what way does PGK1 affect cancer?

PGK1 is often upregulated in cancer cells, aiding their rapid growth and proliferation by enhancing glycolysis, known as the Warburg effect.

Can PGK1 be a target for cancer treatment?

Research suggests that targeting PGK1 could potentially be a strategy for cancer therapy by disrupting the energy production in cancer cells.

Are there any PGK1 inhibitors or activators in development for clinical use?

Several compounds that modulate PGK1 activity are being studied, but none have reached widespread clinical use yet.

Are there diagnostic uses for PGK1 in cancer?

Yes, elevated levels of PGK1 have been identified in certain cancers, making it a potential biomarker for cancer detection and prognosis.