Recombinant Human Plasminogen
Cat.No. : | PLG-66H |
Product Overview : | Recombinant Human Angiostatin produced inPichia Pastorisis a single, non-glycosylated polypeptide chain having a molecular mass of 29711 Dalton. |
- Specification
- Gene Information
- Related Products
- Download
Cat. No. : | PLG-66H |
Description : | RHuAng K1-3 is a proteolytic fragment of plasminogen containing the first three kringle structures. A specific inhibitor of endothelial cell growth and angiogenesis. More active relative to kringles 1-4. rHuAng K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. The rHuAng K1-3 is purified by proprietary chromatographic techniques. |
Source : | Pichia Pastoris. |
Physical Appearance : | Sterile Filtered lyophilized powder. |
Purity : | Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
Formulation : | Lyophilized from a (1mg/ml) solution in containing 0.5X PBS. |
Solubility : | It is recommended to reconstitute the lyophilized rHuAng K1-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Biological Activity : | Inhibitory activity (ED50=70 nm) relative to Kringles 1-4 (ED50=135 nm). Kringles 1-3 acts as inhibitor of endothelial cell growth and angiogenesis. |
Endotoxin : | Less than 0.1 ng/µg (IEU/µg) of rHuAng K1-3. |
Protein Content : | Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 2.08 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of rHuAng K1-3 as a Reference Standard. |
Storage : | Lyophilized rHuAng K1-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuAng K1-3 should be stored at 4°Cbetween 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Gene Name : | PLG plasminogen [ Homo sapiens ] |
Synonyms : | PLG; plasminogen; DKFZp779M0222; Plasminogen; Plasmin heavy chain A;Activation peptide; Angiostatin; Plasmin heavy chainA, short form; Plasmin light chain B;EC 3.4.21.7; OTTHUMP00000017544 |
Gene ID : | 5340 |
mRNA Refseq : | NM_000301 |
Protein Refseq : | NP_000292 |
MIM : | 173350 |
UniProt ID : | P00747 |
Chromosome Location : | 6q26 |
Pathway : | Complement and coagulation cascades; Neuroactive ligand-receptor interaction; Diabetes pathways; Hemostasis; Signaling by PDGF |
Function : | calcium ion binding; apolipoprotein binding; peptidase activity; serine-type endopeptidase activity |
Products Types
◆ Recombinant Protein | ||
PLG-1186P | Recombinant Pig PLG Protein, His-tagged | +Inquiry |
PLG-3290R | Recombinant Rhesus Macaque PLG Protein, His (Fc)-Avi-tagged | +Inquiry |
PLG-134H | Recombinant Human PLG Protein, His (Fc)-Avi-tagged | +Inquiry |
Plg-2673C | Recombinant Chicken Plg Protein, His-tagged | +Inquiry |
PLG-6846M | Recombinant Mouse PLG Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Native Protein | ||
PLG-1867B | Native Bovine PLG Protein | +Inquiry |
PLG-268B | Active Native Bovine glu-Plasminogen | +Inquiry |
Plg-5356M | Native Mouse Plg protein | +Inquiry |
◆ Lysates | ||
PLG-3109HCL | Recombinant Human PLG 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (7)
Ask a questionPLG has been investigated as a potential diagnostic and prognostic biomarker for various diseases. For instance, altered PLG levels have been observed in cancer patients, serving as a potential marker for tumor progression and prognosis. Additionally, PLG has been implicated in cardiovascular diseases, where its levels can reflect the severity of certain conditions, such as thrombosis. Moreover, PLG may have utility as a biomarker in inflammatory disorders and neurological diseases. Further research is needed to validate the clinical utility of PLG as a biomarker and develop specific assays for its detection.
Dysregulation of PLG has been associated with several pathological conditions. Decreased PLG levels or impaired activation can lead to impaired fibrinolysis, resulting in the formation of excessive blood clots. On the other hand, increased PLG activation or excessive Plasmin activity can promote tissue degradation, contributing to diseases such as chronic inflammation, cancer metastasis, and tissue damage. Moreover, PLG has been implicated in the pathogenesis of neurodegenerative disorders and cardiovascular diseases. Understanding the mechanisms underlying PLG dysregulation is crucial for designing therapeutic strategies to counteract its pathological implications.
PLG-targeted therapeutic strategies have shown promise in various diseases. For example, agents that enhance PLG activation, such as tPA and uPA, have been employed to promote fibrinolysis and dissolve blood clots in conditions such as heart attacks and ischemic strokes. Conversely, inhibitors of PLG activation, such as PAIs, can be used to prevent excessive fibrinolysis and subsequent bleeding complications. Additionally, targeting PLG receptors or manipulating PLG interactions with other molecules may offer potential therapeutic avenues. However, further research is required to optimize these strategies and assess their efficacy and safety in clinical settings.
The PLG protein, also known as Plasminogen, is a single-chain glycoprotein composed of 790 amino acids. It consists of several domains, including a signal peptide, five kringle domains (K1-K5), and a serine protease domain. The signal peptide mediates protein secretion, while the kringle domains have numerous functions, such as binding to receptors and ligands. The serine protease domain is responsible for the conversion of Plasminogen to active Plasmin, a crucial enzyme in fibrinolysis and extracellular matrix remodeling processes.
PLG plays a vital role in several physiological processes. Its primary function is as a precursor to Plasmin, which is involved in the degradation of blood clots (fibrinolysis). Plasmin also participates in tissue remodeling, wound healing, and cell migration. Additionally, PLG has been implicated in immune responses, angiogenesis, and cell adhesion. Its ability to interact with various receptors and bind to components of the extracellular matrix highlights its diverse and important functions in maintaining normal physiological homeostasis.
PLG, also known as plasminogen, plays a crucial role in the fibrinolytic system. Its main biological function is to be converted into plasmin, an enzyme that breaks down fibrin clots. Plasminogen is activated by tissue plasminogen activator (tPA), which cleaves it into active plasmin. Plasmin then degrades fibrin clots, preventing the formation of thrombi and promoting the dissolution of existing blood clots. Additionally, PLG is involved in various physiological processes, such as wound healing, tissue remodeling, cell migration, and inflammation.
The activation of PLG is tightly regulated to prevent undesired fibrinolysis. Activation can occur through two main pathways: the tissue-type Plasminogen Activator (tPA)-dependent pathway and the urokinase-type Plasminogen Activator (uPA)-dependent pathway. In the tPA-dependent pathway, tPA binds to a specific receptor on the cell surface and catalyzes the conversion of PLG to Plasmin. The uPA-dependent pathway involves uPA binding to a receptor, followed by the activation of PLG. Additionally, various regulatory proteins, such as Plasminogen Activator Inhibitors (PAIs), control PLG activation by inhibiting the activity of tPA and uPA.
Customer Reviews (3)
Write a reviewThe tightly sealed packaging of this protein reagent ensures optimal preservation of its activity.
I highly recommend this reagent for its simplified experimental workflow, saving time and experimental costs.
Catering to diverse experimental needs, its outstanding performance facilitates diversity in scientific research.
Ask a Question for All PLG Products
Required fields are marked with *
My Review for All PLG Products
Required fields are marked with *
Inquiry Basket