Active Recombinant Human LBP, His-tagged
Cat.No. : | LBP-152H |
Product Overview : | The Lipopolysaccharide Binding Protein is produced from human LBP transfected CHO-cells in serum free medium. Before transfection the complete human LBP-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. The recombinant Human LBP was purified by his-tag with metal affinity purification with Talon and controlled by SDS page.Attention: His-tag has no protease site and can not be split off. |
- Specification
- Gene Information
- Related Products
Description : | The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). |
Source : | Chinese Hamster Ovarian Cells (CHO). |
Species : | Human |
Tag : | His |
Form : | Sterile Filtered White lyophilized (freeze-dried) powder. Recombinant Human LBP was lyophilized from a protein solution (0.25 mg/ml) containing phosphate-buffered saline, pH 7.2. |
Bio-activity : | Up to 0.2 µg/ ml LBP mediates binding of FITC-LPS (0.5µg/ml) to CD14+CHO transfectants (FACS). |
Molecular Mass : | On SDS-PAGE commassie blue stained gel, the 95% purified recombinant protein shows a band at 58 kDa. |
Stability : | Lyophilized LBP Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LBP should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles. |
Reconstitution : | Every 10µg of recombinant human LBP should be reconstituted using 40µl of sterile H2O. The solution can be than diluted with phosphate-buffered saline or other buffers. |
Gene Name : | LBP lipopolysaccharide binding protein [ Homo sapiens (human) ] |
Official Symbol : | LBP |
Synonyms : | LBP; lipopolysaccharide binding protein; MGC22233; lipopolysaccharide-binding protein; LPS-binding protein; OTTHUMP00000030965 |
Gene ID : | 3929 |
mRNA Refseq : | NM_004139 |
Protein Refseq : | NP_004130 |
MIM : | 151990 |
UniProt ID : | P18428 |
Chromosome Location : | 20q11.23 |
Pathway : | IL6-mediated signaling events; NF-kappa B signaling pathway; Toll Like Receptor 4 (TLR4) Cascade |
Function : | lipopolysaccharide binding; lipoteichoic acid binding; protein binding; receptor binding |
Products Types
◆ Recombinant Protein | ||
LBP-007H | Recombinant Human LBP Protein, His-tagged | +Inquiry |
Lbp-5007M | Recombinant Mouse Lbp Protein, His (Fc)-Avi-tagged | +Inquiry |
LBP-4447H | Recombinant Human LBP Protein, His (Fc)-Avi-tagged | +Inquiry |
Lbp-5009M | Recombinant Mouse Lbp Protein, His (Fc)-Avi-tagged | +Inquiry |
LBP-4446H | Recombinant Human LBP Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
LBP-1853HCL | Recombinant Human LBP cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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