Recombinant Human Microphthalmia-associated Transcription Factor

• Cat.No.: MITF-161H
• Product Overview: Recombinant Human MITF protein expressed inE.coliencodes amino acids 170-279 and has a molecular mass of 38 kDa.

Specification

• Cat. No.: MITF-161H
• Description: Mi. is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transtripotion factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells.There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kDa and 56kDa, while the longer Mi form runs as a cluster of bands at 60-70kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart.
• Source: Recombinant Human MITF protein encoding a.a. 170-279 expressed inE.coli.
• Formulation: Recombinant Human MITF protein at 100µg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% Glycerol.
• Application And Suggested Dilutions: •ELISA •Inhibition Assays •Western Blotting
• Characterization: On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 38 kDa.
• Storage & Stability: Store vial at -20°Cto -80°C. When stored at the recommended temperature, this protein is stable for 12 months.

Gene Information

• Gene Name: MITF microphthalmia-associated transcription factor [ Homo sapiens ]
• Synonyms: MITF; microphthalmia-associated transcription factor; MI; WS2A; bHLHe32; homolog of mouse microphthalmia; MI; OTTHUMP00000195123; OTTHUMP00000195140
• Gene ID: 4286
• mRNA Refseq: NM_000248
• Protein Refseq: NP_000239
• MIM: 156845
• UniProt ID: O75030
• Chromosome Location: 3p14.1-p12.3
• Pathway: Melanogenesis; Melanoma; Pathways in cancer
• Function: RNA polymerase II transcription factor activity, enhancer binding; chromatin binding; transcription activator activity; transcription activator activity

Reviews

01/26/2023

They consistently stay at the forefront of scientific advancements and customer feedback, allowing them to refine the MITF protein based on emerging research trends.

08/15/2022

This high-quality protein serves as an invaluable tool in unraveling the intricate mechanisms governed by MITF, enabling me to gain deeper insights into its functions and implications.

12/09/2016

An outstanding aspect of the manufacturer of the MITF protein is their commitment to providing excellent technical support.

Q&As

How does MITF contribute to the metastasis of melanoma?

MITF is involved in the regulation of genes associated with invasion and metastasis in melanoma cells. Dysregulation of MITF can promote the metastatic potential of melanoma, making it an important factor to consider in clinical management.

Are there diagnostic tools that utilize MITF as a marker for melanoma?

While MITF itself may not be used as a standalone diagnostic marker, its expression levels, along with other molecular markers, can be integrated into diagnostic tools to provide a more comprehensive assessment of melanoma.

How does MITF contribute to the resistance of melanoma to immunotherapy?

MITF has been associated with the downregulation of immune-related genes in melanoma cells, potentially contributing to resistance against immunotherapy. Understanding this interaction is crucial for developing strategies to overcome resistance.

In what ways does MITF interact with other signaling pathways in melanoma cells?

MITF interacts with various signaling pathways, including the MAPK pathway. Understanding these interactions is crucial for unraveling the complex molecular networks involved in melanoma and for developing targeted therapeutic strategies.

How can MITF expression levels be measured in clinical settings?

MITF expression levels can be assessed through various laboratory techniques, including immunohistochemistry (IHC) on tissue samples. This provides valuable information for clinicians regarding the status of MITF in melanoma patients.