Recombinant Human tripartite motif-containing 21

• Cat.No.: TRIM21-196H
• Product Overview: Recombinant Human RO-52 produced inE.Coliis a single, non-glycosylated, polypeptide chain. The protein has a molecular mass of 52kDa. The RO-52 is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: TRIM21-196H
• Description: Calreticulin is a multifunctional protein that binds Ca2+ ions (a second messenger molecule in signal transduction), rendering it inactive. The Ca2+ is bound with low affinity, but high capacity, and can be released on a signal (see inositol triphosphate). Calreticulin is located in storage compartments associated with the endoplasmic reticulum.
• Source: Escherichia Coli.
• Amino Acid Sequence: MASAARLTMMWEEVTCPICLDPFVE PVSIECGHSFCQECISQVGKGGGSV CAVCRQRFLLKNLRPNRQLANMVNN LKEISQEAREGTQGERCAVHGERLH LFCEKDGKALCWVCAQSRKHRDHAM VPLEEAAQEYQEKLQVALGELRRKQ ELAEKLEVEIAIKRADWKKTVETQK SRIHAEFVQQKNFLVEEEQRQLQEL EKDEREQLRILGEKEAKLAQQSQAL QELISELDRRCHSSALELLQEVIIV LERSESWNLKDLDITSPELRSVCHV PGLKKMLRTCAVHITLDPDTANPWL ILSEDRRQVRLGDTQQSIPGNEERF DSYPMVLGAQHFHSGKHYWEVDVTG KEAWDLGVCRDSVRRKGHFLLSSKS GFWTIWLWNKQKYEAGTYPQTPLHL QVPPCQVGIFLDYEAGMVSFYNITD HGSLIYSFSECAFTGPLRPFFSPGF NDGGKNTAPLTLCPLNIGSQGSTDY .
• Physical Appearance: Sterile Filtered clear solution.
• Formulation: The protein solution (1mg/ml) contains 6M Urea, 500mM NaCl and 10mM Tris pH8.0.
• Purity: Greater than 90.0% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Protein Content: Protein quantitation was carried out by using 0.25 -2.0 mg/ml Bradford assay vs. BSA.
• Western Blot: Strongly reactive with human anti RO/SS-A.
• Stability: Lyophilized RO-52 although stable at 4°Cfor 3 weeks, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: TRIM21 tripartite motif-containing 21 [ Homo sapiens ]
• Synonyms: TRIM21; tripartite motif-containing 21; SSA; RO52; SSA1; RNF81; SSA1; Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro);52 kDa Ro protein;Sjoegren syndrome type A antigen; SS-A; Ro(SS-A); 52 kDa ribonucleoprotein autoantigen Ro/SS-A; Tripartite motif-containing protein 21; RING finger protein 81; TRIM21 ; Ro(SS-A); SS-A; 52 kDa ribonucleoprotein autoantigen Ro/SS-A; Sicca syndrome antigen A; Sjoegren syndrome type A antigen; Sjogren syndrome antigen A1 (52kDa, ribonucleoproteinautoantigen SS-A/Ro); tripartite motif protein 21; Tripartite motif-containing 21
• Gene ID: 6737
• mRNA Refseq: NM_003141
• Protein Refseq: NP_003132
• MIM: 1099092
• UniProt ID: P19474
• Chromosome Location: 11p15.5-p15.3
• Pathway: Systemic lupus erythematosus
• Function: DNA binding;RNA binding;metal ion binding;protein binding;zinc ion binding