Active Recombinant Human Oncostatin M

• Cat.No.: OSM-131H
• Product Overview: Recombinant Human Oncostatin M was expressed in modifiedhuman 293 cells.

Specification

• Cat. No.: OSM-131H
• Description: Oncostatin M (OSM) is a member of the IL-6 family of cytokines, which includes IL-6, IL-11, LIF, ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1) and cardiotropin-like cytokine. Structurally, OSM is a 196 amino acid monomeric glycoprotein with two potential N-linked glycosylation sites and five cysteine residues, four of which form disulfide bonds. OSM is a growth regulator, in particular with respect to human fibroblasts and vascular smooth muscle cells. Additionally, OSM stimulates the production of IL-6, G-CSF and GM-CSF from endothelial cells and hence plays a role in hematopoiesis and myelopoiesis. OSM mediates the differentiation of megakaryocytes, as well as the regulation of cholesterol metabolism, bone formation and wound healing. OSM has also been shown to play a role in both pro and anti-inflammatory actions. OSM may also be involved in multiple sclerosis (MS), as it is present in MS lesions in microglial cells and infiltrating leukocytes.
• Source: human 293 cells.
• Theoretical Sequence: AAIGSCSKE YRVLLGQLQKQ TDLMQDTSRL LDPYIRIQGLD VPKLREHCRE RP G AFPSEET LRGLGRR GFLQTLNA TLGCVLHR LADLEQRL PKAQDLER SGLNIEDL EKLQMARP NILGLRNN IYCMAQLL DNSDTAEP TKAGRGAS QPPTPTPA S D A F QRK LEGCRFL HGYHRFMHSVGRVFSKWGESPNRSRRHSPHQALRKGVRR.
• Molecular Mass: Oncostatin M migrates as a broad band between 25 and 40 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with unmodified Oncostatin M that has a predicted molecular mass of 23.7 kDa.
• PI: Oncostatin M separates into a number of isoforms with a pI between 5.5 and 10 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified Oncostatin M that has a predicted pI of 9.97.
• % Carbohydrate: purified Oncostatin M consists of 0-40% carbohydrate by weight.
• Glycosylation: Oncostatin M contains O-linked oligosaccharides.
• Purity: >95%, as determined by SDS-PAGE and visualized by silver stain.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C.
• Activity: The ED₅₀of Oncostatin M is typically 0.25-0.50 ng/ml as measured in a cell proliferation assay using the human growth factor-dependent TF-1 cell line.

Gene Information

• Gene Name: OSM oncostatin M [ Homo sapiens ]
• Synonyms: oncostatin M; MGC20461; OSM; MGC20461
• Gene ID: 5008
• mRNA Refseq: NM_020530
• Protein Refseq: NP_065391
• MIM: 165095
• UniProt ID: P13725
• Chromosome Location: 22q12.2
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway
• Function: cytokine activity; growth factor activity; oncostatin-M receptor binding

Reviews

11/25/2020

Outstanding protein-protein interaction studies, vital for our projects.

03/02/2020

High-quality protein characterization, fundamental for our experiments.

02/27/2019

Expertise in peptide synthesis, accelerates our peptide-based research.

Q&As

What is the impact of OSM on cancer development and progression, particularly in terms of tumor microenvironment modulation?

OSM can influence cancer progression by altering the tumor microenvironment and affecting tumor cell behavior.

How does OSM (Oncostatin M) regulate inflammatory and immune responses in the body?

OSM plays a key role in regulating inflammatory and immune responses, modulating cytokine production and immune cell activity.

How does OSM contribute to wound healing and tissue regeneration processes?

OSM aids in wound healing and tissue regeneration, promoting cell growth and repair mechanisms.

What role does OSM play in the pathogenesis of autoimmune diseases like rheumatoid arthritis?

OSM is implicated in autoimmune diseases, contributing to inflammation and joint damage in conditions like rheumatoid arthritis.

How does OSM influence liver fibrosis and hepatic diseases?

OSM is involved in liver fibrosis, impacting the development and progression of hepatic diseases.

What are the mechanisms by which OSM modulates cellular growth, differentiation, and apoptosis?

OSM regulates cellular processes like growth, differentiation, and apoptosis, affecting various tissue functions.

How do alterations in OSM expression or signaling affect cardiovascular health and disease?

Changes in OSM signaling can impact cardiovascular health, influencing disease development and progression.