Recombinant Human Hepatocyte Growth Factor
Cat.No. : | HGF-543H |
Product Overview : | Recombinant Human HGF produced inBaculovirusis a heterodimer, non-glycosylated, polypeptide chain consisting an -chain of 463 amino acids and -chain of 234 having a total molecular mass of 80017 Dalton. HGF is purified by proprietary chromatographic techniques. |
- Specification
- Gene Information
- Related Products
Cat. No. : | HGF-543H |
Description : | Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3 and GM-CSF to stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis. |
Source : | Insect Cells. |
Amino Acid Sequence : | Agrees with the sequence of native human HGF. |
Dimers And Aggregates : | Less than 1% as determined by silver-stained SDS-PAGE gel analysis. |
Purity : | Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
Physical Appearance : | Sterile Filtered White lyophilized (freeze-dried) powder. |
Biological Activity : | Recombinant Human Hepatocyte Growth Factor is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of monkey 4MBr-5 indicator cells was found to be 20-40 ng/ml. |
Endotoxin : | Less than 0.1ng/µg (IEU/µg) of HGF. |
Protein Content : | Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.83 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of HGF as a Reference Standard |
Formulation : | The sterile protein powder is lyophilized with no additives. |
Solubility : | It is recommended to reconstitute the lyophilized HGF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Stability : | Lyophilized HGF although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution HGF should be stored at 4℃between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
Gene Name : | HGF hepatocyte growth factor (hepapoietin A; scatter factor) [ Homo sapiens ] |
Synonyms : | HGF; hepatocyte growth factor (hepapoietin A; scatter factor); SF; HGFB; HPTA; F-TCF; DFNB39; hepatocyte growth factor; scatter factor; hepatopoietin A; OTTHUMP00000206710; lung fibroblast-derived mitogen; fibroblast-derived tumor cytotoxic factor; deafness, autosomal recessive 39; Hepatopoeitin-A; Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain |
Gene ID : | 3082 |
mRNA Refseq : | NM_000601 |
Protein Refseq : | NP_000592 |
MIM : | 142409 |
UniProt ID : | P14210 |
Chromosome Location : | 7q21.1 |
Pathway : | Cytokine-cytokine receptor; Focal adhesion; Melanoma; Pathways in cancer; Renal cell carcinoma; Hemostasis |
Function : | catalytic activity; growth factor activity; protein binding; protein heterodimerization activity; serine-type endopeptidase activity |
Products Types
◆ Recombinant Protein | ||
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Hgf-8664M | Active Recombinant Mouse Hgf protein(Met1-Leu728), His-tagged | +Inquiry |
HGF-3402M | Active Recombinant Mouse HGF protein(Met1-Leu728) | +Inquiry |
Hgf-257M | Recombinant Mouse Hgf Protein | +Inquiry |
Hgf-1237R | Recombinant Rat Hgf Protein, GST-tagged | +Inquiry |
◆ Native Protein | ||
HGF-29231TH | Native Human HGF | +Inquiry |
HGF-38P | Native Porcine HGF | +Inquiry |
◆ Lysates | ||
HGF-001HCL | Recombinant Human HGF cell lysate | +Inquiry |
HGF-1210MCL | Recombinant Mouse HGF cell lysate | +Inquiry |
HGF-1077CCL | Recombinant Cynomolgus HGF cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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