Recombinant Human Heparanase

• Cat.No.: HPSE-213H
• Product Overview: Recombinant Human Heparanase-1 is produced as a latent 65 kDa precursor and proteolytically processed to its active form inCHO cell.

Specification

• Cat. No.: HPSE-213H
• Description: Heparanase is an endo β-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasation of tumor cells and inflammatory leukocytes. Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis. Heparanase is a heterodimer comprised of a 50 kDa subunit harboring the active site and a 8 kDa subunit. Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor chance of survival.
• Source: CHO.
• Concentration: 1µg /ml.
• Buffer: LDS-PAGE buffer
• Application: Positive control for western blot analysis.
• Storage: Store at –20 ℃, avoid repeated freeze-thaw cycles.
• Publication:
  Functional and structural characterization of a heparanase (2015)
  Metalloglycomics of tris(2,2′-bipyridyl) cobalt and ruthenium compounds (2022)

Gene Information

• Gene Name: HPSE heparanase [ Homo sapiens ]
• Synonyms: HPSE; heparanase; HPA; HPA1; HPR1; HPSE1; HSE1; heparanase-1; heparanase exon10-deletion; heparanase exon 9&10 deletion;EC=3.2.-.-; Heparanase 8 kDa subunit; Heparanase 50 kDa subunit
• Gene ID: 10855
• mRNA Refseq: NM_001098540
• Protein Refseq: NP_001092010
• MIM: 604724
• UniProt ID: Q9Y251
• Chromosome Location: 4q21.3
• Pathway: Glycosaminoglycan degradation; Metabolic pathways
• Function: beta-glucuronidase activity; calcium ion binding; hydrolase activity, acting on glycosyl bonds; magnesium ion binding

Reviews

12/08/2019

The stability of HPSE was still very good under different temperature conditions, indicating that it has excellent thermal stability.

05/19/2019

Beacuse high efficiency and wide storage conditions of proteins make the experimental process more convenient and greatly improve the efficiency of scientific research.

Q&As

What is the role of HPSE protein in tumor immune evasion?

By regulating the tumor microenvironment and the function of immune cells, HPSE protein participates in the process of tumor immune evasion.

Does HPSE protein have potential applications in tumor immunotherapy?

Yes, the mechanism by which HPSE proteins act in relation to tumor immunotherapy has potential applications in this regard.

How about the fuction of HPSE protein in liver fibrosis?

The protein plays an important role in liver fibrosis and participates in the regulation of liver tissue fibrosis through mechanisms such as collagen degradation.

How does HPSE protein participate in tumor invasion and metastasis?

HPSE protein is involved in regulating tumor invasion and metastasis by degrading the basement membrane and promoting cytotoxicity.

What is the role of HPSE protein in collagen degradation, tissue repair and regeneration?

The protein plays an important role in collagen degradation, tissue repair and regeneration, participates in epithelial-mesenchymal transformation processes, and affects biological processes such as cell migration and remodeling.

What is the role of HPSE protein in inflammatory response?

HPSE proteins play an important role in the inflammatory response by participating in mechanisms such as extracellular matrix degradation and mediating cytokine production and release.