Active Recombinant Human FCGR2A protein(167 Arg), His-tagged

• Cat.No.: FCGR2A-649H
• Product Overview: Recombinant Human FCGR2A protein (Met1-Ile218) (P12318-1, with Natural variant His 167 Arg), fused with a C-terminal polyhistidine tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.5
  Normally 5 % trehalose, 5 % mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to bind biotinylated human IgG2 in functional ELISA.
  2. Loaded Anti-CD32A Antibody, Mouse Monoclonal on AMC Biosensor, can bind CD32A Protein, Human, Recombinant (167 Arg, His Tag) with an affinity constant of 6.8 nM as determined by Octet RED System.
• Molecular Mass: The recombinant human FCGR2A consists of 196 amino acids after removal of the signal peptide and predicts a molecular mass of 22 kDa. It migrates as an approximately 30 kDa protein due to glycosylation by SDS-PAGE under reducing conditions.
• Protein length: Met1-Ile218
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FCGR2A Fc fragment of IgG, low affinity IIa, receptor (CD32) [ Homo sapiens ]
• Official Symbol: FCGR2A
• Synonyms: FCGR2A; CD32; CD32A; CDw32; IGFR2; fcRII-a; fc-gamma-RIIa; FCG2; FcGR; FCGR2; FCGR2A1; MGC23887; MGC30032
• Gene ID: 2212
• mRNA Refseq: NM_001136219.1
• Protein Refseq: NP_001129691.1
• MIM: 146790
• UniProt ID: P12318